Mouse NCAM-1/CD56 Antibody Summary
Leu20-Thr711
Accession # P13595
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse NCAM‑1/CD56 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Mouse NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB7820) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for NCAM-1/CD56 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of NCAM‑1/CD56 in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Rat Anti-Mouse NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB7820, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
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Detection of NCAM‑1/CD56 in Mouse Cerebellum. NCAM‑1/CD56 was detected in immersion fixed paraffin-embedded sections of mouse cerebellum using Rat Anti-Mouse NCAM‑1/CD56 Monoclonal Antibody (Catalog # MAB7820) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and neuropils. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of NCAM‑1/CD56 in Mouse Cortex. NCAM‑1/CD56 was detected in immersion fixed paraffin-embedded sections of mouse cortex using Rat Anti-Mouse NCAM‑1/CD56 Monoclonal Antibody (Catalog # MAB7820) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and neuropils. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NCAM-1/CD56
NCAM-1 (Neural adhesion molecule-1; also CD56) is a 120-190 kDa glycoprotein member of the Ig Superfamily. It is expressed on multiple cell types, both in the embryo and adult. Here, it serves as both an adhesion molecule and a receptor for multiple ligands, including as FGFR, PDGF, GDNF and agrin. On the cell surface, it is a cis-oriented homodimer that can form homodimers in-trans with other cis-homodimers. In the embryo, NCAM-1 is polysialylated (PolySia), and shows a MW of 200-220 kDa in SDS-PAGE. This polysialylation reduces the ability of NCAM-1 to dimerize. Mature mouse NCAM-1 is a 1096 amino acid (aa) type I transmembrane (TM) protein (aa 20-1115). It possesses a 692 aa extracellular region (aa 20-711) and a 386 aa cytoplasmic domain. The extracellular region contains five consecutive C2-type Ig-like domains (aa 20-492) followed by two FN type-III domains (aa 497-692). Multiple splice variants exist. There is a 140 kDa TM variant that shows a deletion of aa 810-1076, and a 120 kDa variant that is GPI-linked and shows a 24 aa substitution for aa 702-1115. A third potential variant contains a five aa substitution for aa 601-1115. Over aa 20-711, mouse NCAM-1 shares 99% and 95% aa identity with rat and human NCAM-1, respectively.
Product Datasheets
Citations for Mouse NCAM-1/CD56 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Diminished expression of major histocompatibility complex facilitates the use of human induced pluripotent stem cells in monkey
Authors: X Wang, M Lu, X Tian, Y Ren, Y Li, M Xiang, S Chen
Stem Cell Res Ther, 2020-08-03;11(1):334.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The prostaglandin E2 receptor EP3 controls CC-chemokine ligand-mediated neuropathic pain induced by mechanical nerve damage
Authors: EM Treutlein, K Kern, A Weigert, N Tarighi, CD Schuh, RM Nüsing, Y Schreiber, N Ferreirós, B Brüne, G Geisslinge, S Pierre, K Scholich
J. Biol. Chem., 2018-05-11;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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