Mouse Neuropoietin/NP Antibody

Catalog #: AF2709 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2709
AF2709-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Neuropoietin/NP Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Neuropoietin/NP in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Neuropoietin/NP
Ala23-Ala204
Accession # P83714

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Neuropoietin/NP (Catalog # 2709-NP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropoietin/NP

Neuropoietin (NP; also known as cardiotrophin-2) is a 22 kDa member of the IL-6 family of cytokines. Considered to be the product of a gene duplication event involving cardiotrophin-1 (CT-1), it helps to define a subfamily within the IL-6 family that includes CT-1, CLC and CTNF. Mouse neuropoietin is synthesized as a 204 amino acid (aa) precursor that contains a 22 aa signal sequence and a 192 aa mature segment. The secreted molecule is characterized by the presence of four alpha ‑helices, typical of hematopoietic superfamily molecules. Mature mouse neuropoietin shares 88%, 90% and 95% aa identity to chimpanzee, canine and rat neuropoietin, respectively. The human gene is suggested to have evolved towards a pseudogene, a point of interest in that neuropoietin is reported to signal through the CNTF complex (i.e., gp130, CNTF R alpha and LIF R). NP will mediate motor neuron survival, and appears to be selectively expressed in the embryo by tissues involved with nervous system development (1).

References