Mouse NKG2C/CD159c Antibody

Catalog #: MAB1382 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB1382-100
MAB1382-SP

Detection of NKG2C/CD159c in HEK293 Human Cell Line Transfected with Mouse NKG2C/CD159c and eGFP by Flow Cytometry.

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All NKG2C/CD159c Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse NKG2C/CD159c Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse NKG2C/CD159c in direct ELISAs. In Flow Cytometry, no cross-reactivity with mouse NKG2A/CD159a is observed.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2098A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse NKG2C/CD159c synthetic peptide
Accession # NP_034783

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NKG2C/CD159c in HEK293 Human Cell Line Transfected with Mouse NKG2C/CD159c and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse NKG2C/CD159c or (B) mouse NKG2A/CD159a and eGFP was stained with Rabbit Anti-Mouse NKG2c/CD159c Monoclonal Antibody (Catalog # MAB1382) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2C/CD159c

NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8⁺ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8⁺ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C⁺ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12).

References

Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
Tassi I. et al. (2006) Immnunol Rev. 214:92.
Lanier, L.L. (2008) Nat. Immunol. 9:495.
Schleinitz, N. et al. (2010) Immunology. 174:2878.
Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
Braud, V.M. et al. (1998) Nature. 391:795.
Lanier, L.L. (1998) Immunity 8:693.
Vance, R.E. et al. (1999) J Exp Med 190:1801.
Kaiser B.K. et al. (2005) J Immunol 174:2878.
Bellón T. et al. (1999) J Immunol 162:3996.
Pupuleku A. et al. (2017) Front Immunol. 8:1317.
Hammer Q. et al. (2018) Nat Immunol. 19:453.

Long Name

Natural Killer G2C

Entrez Gene IDs

3822 (Human); 16642 (Mouse)

Alternate Names

CD159 antigen-like family member C; CD159c antigen; CD159c; killer cell lectin-like receptor subfamily C, member 2; KLRC2; MGC138244; NK cell receptor C; NKG2-C type II integral membrane protein; NKG2C; NKG2-C; NKG2CNKG2-C-activating NK receptor