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Mouse NKp46/NCR1 Antibody

Catalog #: AF2225
33 Citations 1 Review Datasheet / COA / SDS
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AF2225
AF2225-SP
Mouse NKp46/NCR1 Antibody Induces IFN-gamma Secretion in Activated Mouse NK Cells.
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Citations (33)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse NKp46/NCR1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse NKp46/NCR1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) NKp46 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NKp46/NCR1
Glu22-Asn255
Accession # Q8C567
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse NKp46/NCR1 Fc Chimera (Catalog # 2225-NK)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
3-15 µg/mL
See below
Agonist Activity
0.4-2.4 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Agonist Activity Mouse NKp46/NCR1 Antibody Induces IFN-? Secretion antibody in Activated Mouse NK Cells. View Larger

Mouse NKp46/NCR1 Antibody Induces IFN-gamma Secretion in Activated Mouse NK Cells. Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody induces IFN-? secretion in mouse natural killer (NK) cells activated with 25 ng/mL Recombinant Mouse IL-2 (Catalog # 402-ML) and 25 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML), in a dose-dependent manner, as measured using the Quantikine Mouse IFN-? ELISA Kit (Catalog # MIF00). The ED50 for this effect is typically 0.4-2.4 µg/mL.

Flow Cytometry Detection of NKp46/NCR1 antibody in Mouse DX5/CD49b<SUP>+</SUP>Splenocytes antibody by Flow Cytometry. View Larger

Detection of NKp46/NCR1 in Mouse DX5/CD49b+Splenocytes by Flow Cytometry. Mouse DX5/CD49b+splenocytes were stained with Goat Anti-Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2225, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunohistochemistry NKp46/NCR1 antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

NKp46/NCR1 in Mouse Spleen. NKp46/NCR1 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2225) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKp46/NCR1

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1 (3), is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma (4). Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (5). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (6-8).

References
  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  3. Biassoni, R. et al. (1999) Eur. J. Immunol. 29:1014.
  4. Westgaard, I. et al. (2004) J. Leukoc. Biol. PMID 15356098.
  5. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  6. Arnon, T. et al. (2004) Blood 103:664.
  7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
  8. Mandelboim, O. et al. (2001) Nature 409:1055.
Entrez Gene IDs
9437 (Human); 17086 (Mouse); 100141419 (Porcine); 102115479 (Cynomolgus Monkey)
Alternate Names
CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

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Citations for Mouse NKp46/NCR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. PD-1 regulates ILC3-driven intestinal immunity and homeostasis
    Authors: Jacquelot, N;Xiong, L;Cao, WHJ;Huang, Q;Yu, H;Sayad, A;Anttila, CJA;Baldwin, TM;Hickey, PF;Amann-Zalcenstein, D;Ohashi, PS;Nutt, SL;Belz, GT;Seillet, C;
    Mucosal immunology
    Species: Murine polyomavirus strain A3, Viral
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. An oncolytic virus expressing a full-length antibody enhances antitumor innate immune response to glioblastoma
    Authors: B Xu, L Tian, J Chen, J Wang, R Ma, W Dong, A Li, J Zhang, E Antonio Ch, B Kaur, M Feng, MA Caligiuri, J Yu
    Nature Communications, 2021-10-08;12(1):5908.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Neuroblast senescence in the aged brain augments natural killer cell cytotoxicity leading to impaired neurogenesis and cognition
    Authors: WN Jin, K Shi, W He, JH Sun, L Van Kaer, FD Shi, Q Liu
    Nature Neuroscience, 2020-11-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation
    Authors: FM Kobia, K Preusse, Q Dai, N Weaver, MR Hass, P Chaturvedi, SJ Stein, WS Pear, Z Yuan, RA Kovall, Y Kuang, N Eafergen, D Sprinzak, B Gebelein, EW Brunskill, R Kopan
    PLoS Biol, 2020-10-05;18(10):e3000850.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  5. Corneal dysfunction precedes the onset of hyperglycemia in a mouse model of diet-induced obesity
    Authors: A Hargrave, JA Courson, V Pham, P Landry, S Magadi, P Shankar, S Hanlon, A Das, RE Rumbaut, CW Smith, AR Burns
    PLoS ONE, 2020-09-04;15(9):e0238750.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Antitumor Activity of Amivantamab (JNJ-61186372), an EGFR-cMet Bispecific Antibody, in Diverse Models of EGFR Exon 20 Insertion-Driven NSCLC
    Authors: J Yun, SH Lee, SY Kim, SY Jeong, JH Kim, KH Pyo, CW Park, SG Heo, MR Yun, S Lim, SM Lim, MH Hong, HR Kim, M Thayu, JC Curtin, RE Knoblauch, MV Lorenzi, A Roshak, BC Cho
    Cancer Discov, 2020-05-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Forced expression of CXCL10 prevents liver metastasis of colon carcinoma cells by the recruitment of natural killer cells
    Authors: N Kikuchi, J Ye, J Hirakawa, H Kawashima
    Biol. Pharm. Bull., 2018-11-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice
    Authors: S Nishina, A Yamauchi, T Kawaguchi, K Kaku, M Goto, K Sasaki, Y Hara, Y Tomiyama, F Kuribayash, T Torimura, K Hino
    Cell Mol Gastroenterol Hepatol, 2018-09-11;7(1):115-134.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Pericyte-like spreading by disseminated cancer cells activates YAP and MRTF for metastatic colonization
    Authors: EE Er, M Valiente, K Ganesh, Y Zou, S Agrawal, J Hu, B Griscom, M Rosenblum, A Boire, E Brogi, FG Giancotti, M Schachner, S Malladi, J Massagué
    Nat. Cell Biol., 2018-07-23;20(8):966-978.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Therapeutic Potential of Leelamine, a Novel Inhibitor of Androgen Receptor and Castration-Resistant Prostate Cancer
    Authors: KB Singh, X Ji, SV Singh
    Mol. Cancer Ther., 2018-07-20;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Preclinical, non-genetic models of lung adenocarcinoma: a comparative survey
    Authors: F Janker, W Weder, JH Jang, W Jungraithm
    Oncotarget, 2018-07-17;9(55):30527-30538.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Tumor-derived granzyme B-expressing neutrophils acquire antitumor potential after lipid A treatment.
    Authors: A Martin, C Seignez, C Racoeur, N Isambert, Isambert N, N Mabrouk, Mabrouk N, A Scagliarin, Scagliarini A, S Reveneau, Reveneau S, L Arnould, Arnould L, A Bettaieb, JF Jeannin, Jeannin J, C Paul
    Oncotarget, 2018-06-19;9(47):28364-28378.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. NKp46 Calibrates Tumoricidal Potential of Type 1 Innate Lymphocytes by Regulating TRAIL Expression
    Authors: G Turchinovi, S Ganter, A Bärenwaldt, D Finke
    J. Immunol., 2018-04-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. NK cell heparanase controls tumor invasion and immune surveillance
    Authors: EM Putz, AJ Mayfosh, K Kos, DS Barkauskas, K Nakamura, L Town, KJ Goodall, DY Yee, IK Poon, N Baschuk, F Souza-Fons, MD Hulett, MJ Smyth
    J. Clin. Invest., 2017-06-05;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
    Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
    J. Immunol, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  16. Independent control of natural killer cell responsiveness and homeostasis at steady-state by CD11c+ dendritic cells
    Sci Rep, 2016-12-01;6(0):37996.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  17. Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells
    J Leukoc Biol, 2016-08-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. IL-2 in the tumor microenvironment is necessary for Wiskott-Aldrich syndrome protein deficient NK cells to respond to tumors in vivo
    Sci Rep, 2016-08-01;6(0):30636.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  19. Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival
    Authors: W Rabacal, SK Pabbisetty, KL Hoek, D Cendron, Y Guo, D Maseda, E Sebzda
    Proc Natl Acad Sci USA, 2016-04-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. IL-15 Superagonist-Mediated Immunotoxicity: Role of NK Cells and IFN-gamma.
    Authors: Guo Y, Luan L, Rabacal W, Bohannon J, Fensterheim B, Hernandez A, Sherwood E
    J Immunol, 2015-07-27;195(5):2353-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  21. NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
    Authors: Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D
    J Leukoc Biol, 2015-01-12;97(3):583-98.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC, IHC-P
  22. Antigen dependently activated cluster of differentiation 8-positive T cells cause perforin-mediated neurotoxicity in experimental stroke.
    Authors: Mracsko E, Liesz A, Stojanovic A, Lou W, Osswald M, Zhou W, Karcher S, Winkler F, Martin-Villalba A, Cerwenka A, Veltkamp R
    J Neurosci, 2014-12-10;34(50):16784-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. BCMab1, a monoclonal antibody against aberrantly glycosylated integrin alpha3beta1, has potent antitumor activity of bladder cancer in vivo.
    Authors: Li C, Yang Z, Du Y, Tang H, Chen J, Hu D, Fan Z
    Clin Cancer Res, 2014-07-07;20(15):4001-13.
    Species: Mouse
    Sample Types:
  24. Role of CC chemokine receptor 4 in natural killer cell activation during acute cigarette smoke exposure.
    Authors: Stolberg V, Martin B, Mancuso P, Olszewski M, Freeman C, Curtis J, Chensue S
    Am J Pathol, 2013-12-09;184(2):454-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  25. TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
    Authors: Biroccio A, Cherfils-Vicini J, Augereau A, Pinte S, Bauwens S, Ye J, Simonet T, Horard B, Jamet K, Cervera L, Mendez-Bermudez A, Poncet D, Grataroli R, de Rodenbeeke C, Salvati E, Rizzo A, Zizza P, Ricoul M, Cognet C, Kuilman T, Duret H, Lepinasse F, Marvel J, Verhoeyen E, Cosset F, Peeper D, Smyth M, Londono-Vallejo A, Sabatier L, Picco V, Pages G, Scoazec J, Stoppacciaro A, Leonetti C, Vivier E, Gilson E
    Nat Cell Biol, 2013-06-23;15(7):818-28.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
    Authors: Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL
    PLoS Pathog., 2012-01-12;8(1):e1002481.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46.
    Authors: Gur C, Enk J, Kassem SA, Suissa Y, Magenheim J, Stolovich-Rain M, Nir T, Achdout H, Glaser B, Shapiro J, Naparstek Y, Porgador A, Dor Y, Mandelboim O
    J. Immunol., 2011-08-17;187(6):3096-103.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  28. Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells.
    Authors: Eidenschenk C, Crozat K, Krebs P, Arens R, Popkin D, Arnold CN, Blasius AL, Benedict CA, Moresco EM, Xia Y, Beutler B
    Proc. Natl. Acad. Sci. U.S.A., 2010-05-10;107(21):9759-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  29. Distinct phenotype and function of NK cells in the pancreas of nonobese diabetic mice.
    Authors: Brauner H, Elemans M, Lemos S, Broberger C, Holmberg D, Flodstrom-Tullberg M, Karre K, Hoglund P
    J. Immunol., 2010-02-03;184(5):2272-80.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Functional Assay, IHC
  30. T-cell recruitment and Th1 polarization in adipose tissue during diet-induced obesity in C57BL/6 mice.
    Authors: Strissel KJ, DeFuria J, Shaul ME, Bennett G, Greenberg AS, Obin MS
    Obesity (Silver Spring), 2010-01-28;18(10):1918-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  31. Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model.
    Authors: Luangsay S, Wittamer V, Bondue B, De Henau O, Rouger L, Brait M, Franssen JD, de Nadai P, Huaux F, Parmentier M
    J. Immunol., 2009-10-19;183(10):6489-99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  32. Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells.
    Authors: Kang SJ, Liang HE, Reizis B, Locksley RM
    Immunity, 2008-11-14;29(5):819-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  33. CD11cloB220+ interferon-producing killer dendritic cells are activated natural killer cells.
    Authors: Vosshenrich CA, Lesjean-Pottier S, Hasan M, Richard-Le Goff O, Corcuff E, Mandelboim O, Di Santo JP
    J. Exp. Med., 2007-10-08;204(11):2569-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse NKp46/NCR1 Antibody
By Efstathios Stamatiades on 07/10/2019
Application: ICC/IF Sample Tested: Salivary gland tissue Species: Mouse

PLP-fixed salivary gland cryosections were stained with anti NKp46/NCR1 for 4h at room temperature. After washing, the samples were stained with Donkey anti-goat alexa546 2ndary antibody for 1h at room temperature. NKp46 in red, CD3 in green & Hoechst in blue.