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Mouse OCAM/NCAM2 Antibody

Catalog #: MAB778
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB778
MAB778-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse OCAM/NCAM2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse OCAM/NCAM2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhMCAM, recombinant mouse (rm) NCAM-L1, or rmMAdCAM-1 is observed.
Source
Monoclonal Rat IgG2A Clone # 125101
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse OCAM/NCAM2
Leu20-Gly700 (predicted)
Accession # O35136
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse OCAM

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OCAM/NCAM2

OCAM, also known as neural cell adhesion molecule 2 (NCAM2), belongs to the immunoglobulin superfamily. It exists either as a type I membrane protein or is attached to the membrane via a GPI-anchor.

Long Name
Opioid-binding and Cell Adhesion Molecule/Neural Cell Adhesion Molecule 2
Entrez Gene IDs
4685 (Human); 17968 (Mouse)
Alternate Names
NCAM2; N-CAM-2; NCAM-2; NCAM21MGC51008; neural cell adhesion molecule 2 precursor10; neural cell adhesion molecule 2; OCAM; Rb-8; RNCAM

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Citations for Mouse OCAM/NCAM2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
    Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
    Nat Commun, 2020-10-14;11(1):5171.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. A beta -dependent reduction of NCAM2-mediated synaptic adhesion contributes to synapse loss in Alzheimer’s disease
    Authors: Iryna Leshchyns’ka, Heng Tai Liew, Claire Shepherd, Glenda M. Halliday, Claire H. Stevens, Yazi D. Ke et al.
    Nature Communications
  3. OCAM Regulates Embryonic Spinal Cord Stem Cell Proliferation by Modulating ErbB2 Receptor.
    Authors: Deleyrolle L, Sabourin J, Rothhut B, Fujita H, Guichet P, Teigell M, Ripoll C, Chauvet N, Perrin F, Mamaeva D, Noda T, Mori K, Yoshihara Y, Hugnot J
    PLoS ONE, 2015-04-13;10(4):e0122337.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC

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