Mouse PD-L2/B7-DC Antibody

Name: Mouse PD-L2/B7-DC Antibody
Brand: R&D Systems
SKU: AF1022
Rating: 4.5 (2 reviews)

Catalog #: AF1022
3 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1022
AF1022-SP

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All PD-L2/B7-DC Antibodies

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PD-L2/B7-DC Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse PD-L2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human PD-L2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PD-L2
Leu20-Arg219
Accession # Q9WUL5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse PD-L2 Fc Chimera (Catalog # 1022-PL)

Flow Cytometry

2.5 µg/10⁶ cells

RAW 264.7 mouse monocyte/macrophage cell line treated with LPS

Immunohistochemistry

5-15 µg/mL

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 1 µg/mL of Recombinant Mouse PD-1 (Catalog # 1021-PD) to immobilized Recombinant Mouse PD-L2 Fc Chimera (Catalog # 1022-PL) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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PD‑L2 in Mouse Thymus. PD-L2 was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Goat Anti-Mouse PD-L2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1022) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L2/B7-DC

Mouse Programmed Death Ligand 2 (PD-L2), also named B7DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1-4). Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and B7-H3. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share from 20-40% amino acid (aa) sequence identity. The cloned mouse PD-L2 cDNA encodes a 247 aa type I membrane precursor protein with a putative 20 aa signal peptide, a 199 aa extracellular region containing one V-like and one C-like Ig domain, a 23 aa transmembrane region, and only a 5 aa cytoplasmic domain. The extracellular domains of mouse and human PD-L2 share approximately 72% aa sequence identity. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L1. Mouse PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. PD-L2 is constitutively expressed in lymphoid and non-lymphoid organs (1-4). The expression of PD-L2 is detected on dendritic cells, thymic epithelial cells and IFN-gamma treated monocytes. PD-L2 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, PD-L2 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a co-stimulatory function for the PD-1 ligands on resting T cells has also been reported.

References

Latchman, Y. et al. (2001) Nature Immun. 2:261.
Tseng, B.S-Y. et al. (2001) J. Exp. Med. 193:839.
Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.

Long Name

Programmed Death-1 Ligand-2

Entrez Gene IDs

80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)

Alternate Names

B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; Butyrophilin-like Protein; CD273 antigen; CD273; CD273PD-1 ligand 2; MGC142240; PD-1-ligand 2; PDCD1L2MGC142238; PDCD1LG2; PDL2; PD-L2; PDL2B7DC; PD-L2PDCD1 ligand 2; programmed cell death 1 ligand 2; Programmed death ligand 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse PD-L2/B7-DC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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The Circadian Clock Controls Immune Checkpoint Pathway in Sepsis
Authors: W Deng, S Zhu, L Zeng, J Liu, R Kang, M Yang, L Cao, H Wang, TR Billiar, J Jiang, M Xie, D Tang
Cell Rep, 2018-07-10;24(2):366-378.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice.
Authors: Kasagi S, Kawano S, Okazaki T, Honjo T, Morinobu A, Hatachi S, Shimatani K, Tanaka Y, Minato N, Kumagai S
J. Immunol., 2010-02-05;184(5):2337-47.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Establishment of NOD-Pdcd1-/- mice as an efficient animal model of type I diabetes.
Authors: Wang J, Yoshida T, Nakaki F, Hiai H, Okazaki T, Honjo T
Proc. Natl. Acad. Sci. U.S.A., 2005-08-08;102(33):11823-8.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC