Mouse Persephin Antibody

Catalog #: MAB2479 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2479
MAB2479-SP

All Persephin Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Persephin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Persephin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) AgRP, rmArtemin, rmCripto, recombinant drosophila DPP, recombinant human DPP-6, rhGDNF, rhLAP, rmMIS, rmNodal, rhTGF‑ alpha, rhTGF-beta 1, rhTGF-beta 1.2, rhTGF-beta 2, rhTGF-beta 3, or recombinant amphibian TGF-beta 5 is observed.

Source

Monoclonal Rat IgG_2A Clone # 240839

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Persephin
Ala61-Gly156
Accession # O70300

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Persephin (Catalog # 2479-PS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Persephin

Persephin is a secreted protein belonging to the glial cell line‑derived neurotrophic factor (GDNF) family of the TGF-beta superfamily. It shares 38‑46% amino acid identity with family members GDNF, neurturin and artemin. Persephin is expressed at very low levels in most tissues (1). The 10‑12 kDa mature protein contains several cysteines that are conserved among family members. It circulates as an unglycosylated disulfide-linked homodimer. Mature mouse persephin shares 94%, 81%, 79% and 76% amino acid sequence identity with rat, human, bovine and canine persephin, respectively. Like other GDNF family members, persephin acts through engagement of a glycosylphosphatidylinositol (GPI)-linked GDNF receptor family (GRF) member that signals through the receptor tyrosine kinase RET. Persephin is reported to promote both the survival and growth of central dopaminergic and motor neurons, and kidney development (1). These effects are correlated with the expression patterns of its specific receptor, GFR alpha 4, and RET (2, 3). Functional GFR alpha 4 isoforms are found only in thyroid, adrenal medulla and portions of the central nervous system and include GPI-linked, transmembrane and soluble forms (3, 4). In vitro, persephin promotes survival only in neurons which coexpress GPI-linked GFR alpha 4 with RET (2, 5). This effect does not show a strong correlation to the recruitment of RET in lipid rafts seen with other GDNF family members (6). Disruption of the persephin gene results in mice that are morphologically normal but have more damage and less effective repair after a central nervous system insult simulating a stroke. Microinjection of persephin prior to treatment protects against damage in both wild-type and mutant mouse brains, but surprisingly, high doses of persephin are detrimental (7).

References