Mouse PlGF-2 Antibody

Catalog #: MAB4651 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4651
MAB4651-SP

All PlGF-2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PlGF-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse PlGF-2 in ELISAs. In ELISAs, no cross-reactivity or interference was observed with recombinant mouse (rm) VEGF R1, rmVEGF R2, or rmVEGF R3.

Source

Monoclonal Rat IgG_2A Clone # 62503

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2
Ala24-Pro158
Accession # P49764

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Mouse PlGF-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse PlGF‑2 Biotinylated Antibody (Catalog # BAF465)

Standard: Recombinant Mouse PlGF-2 Protein (Catalog # 465-PL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PlGF-2

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Mouse PlGF-2 encodes a 158 amino acid residue precursor protein. Recombinant mouse PlGF preparations purified from the transfected Sf 21 insect cell conditioned medium contain a mixture of two distinct mature PlGF monomers that are derived from the removal of either a 23 or a 26 residue signal peptide from the amino-terminus of the precursor protein. Mouse PlGF-2 shares 65% amino acid identity with human PlGF-2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high-affinity to Flt-1, but not to Flk-1/KDR.

References