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Mouse Podocalyxin Antibody

Catalog #: AF1556
37 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1556
AF1556-SP
Detection of Mouse Podocalyxin by Western Blot.
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Product Details
Citations (37)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Podocalyxin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
Ser21-Arg402
Accession # Q9R0M4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
D3 mouse embryonic stem cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Podocalyxin antibody by Western Blot. View Larger

Detection of Mouse Podocalyxin by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Podocalyxin antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

Podocalyxin in Mouse Thymus. Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to high endothelial venules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References
  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.
Entrez Gene IDs
5420 (Human); 27205 (Mouse)
Alternate Names
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

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Citations for Mouse Podocalyxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice
    Authors: IS Zaitoun, YS Song, HB Zaitoun, CM Sorenson, N Sheibani
    Cells, 2022-10-21;11(20):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain
    Authors: H Estrada, J Robin, A Özbek, Z Chen, A Marowsky, Q Zhou, D Beck, B le Roy, M Arand, S Shoham, D Razansky
    Science Advances, 2021-12-08;7(50):eabi5464.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures
    Authors: K Gkatzis, P Panza, S Peruzzo, DY Stainier
    Elife, 2021-09-29;10(0):.
    Species: Mouse
    Sample Types: Organoids
    Applications: IHC
  4. A mouse model of prenatal exposure to Interleukin-6 to study the developmental origin of health and disease
    Authors: T Srivastava, T Joshi, DP Heruth, MH Rezaiekhal, RE Garola, J Zhou, VC Boinpelly, MF Ali, US Alon, M Sharma, GB Vanden Heu, P Mahajan, L Priya, Y Jiang, ET McCarthy, VJ Savin, R Sharma, M Sharma
    Scientific Reports, 2021-06-24;11(1):13260.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy
    Authors: C Mao, MY Lee, JR Jhan, AR Halpern, MA Woodworth, AK Glaser, TJ Chozinski, L Shin, JW Pippin, SJ Shankland, JTC Liu, JC Vaughan
    Sci Adv, 2020-05-27;6(22):eaba4542.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Digenic inheritance of mutations in EPHA2 and SLC26A4 in Pendred syndrome
    Authors: M Li, SY Nishio, C Naruse, M Riddell, S Sapski, T Katsuno, T Hikita, F Mizapoursh, FM Smith, LT Cooper, MG Lee, M Asano, T Boettger, M Krueger, A Wietelmann, J Graumann, BW Day, AW Boyd, S Offermanns, SI Kitajiri, SI Usami, M Nakayama
    Nat Commun, 2020-03-12;11(1):1343.
    Species: Mouse
    Sample Types: Cells
    Applications: ICC
  7. Retinoic acid receptor alpha as a novel contributor to adrenal cortex structure and function through interactions with Wnt and Vegfa signalling
    Authors: RM El Zein, AH Soria, JF Golib Dzib, AJ Rickard, FL Fernandes-, B Samson-Cou, I Giscos-Dou, A Rocha, M Poglitsch, CE Gomez-Sanc, L Amar, NB Ghyselinck, A Benecke, MC Zennaro, S Boulkroun
    Sci Rep, 2019-10-11;9(1):14677.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Acute and chronic hypoxia differentially predispose lungs for metastases
    Authors: M Reiterer, R Colaço, P Emrouzneja, A Jensen, H Rundqvist, RS Johnson, C Branco
    Sci Rep, 2019-07-15;9(1):10246.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Evaporative cooling provides a major metabolic energy sink
    Authors: I Kasza, D Adler, DW Nelson, CL Eric Yen, S Dumas, JM Ntambi, OA MacDougald, D Hernando, WP Porter, FA Best, CM Alexander
    Mol Metab, 2019-07-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes
    Authors: R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams
    Nat Commun, 2019-06-27;10(1):2817.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Endothelial cell clonal expansion in the development of cerebral cavernous malformations
    Authors: M Malinverno, C Maderna, A Abu Taha, M Corada, F Orsenigo, M Valentino, F Pisati, C Fusco, P Graziano, M Giannotta, QC Yu, YA Zeng, MG Lampugnani, PU Magnusson, E Dejana
    Nat Commun, 2019-06-24;10(1):2761.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis
    Authors: G Genet, K Boyé, T Mathivet, R Ola, F Zhang, A Dubrac, J Li, N Genet, L Henrique G, L Benedetti, S Künzel, L Pibouin-Fr, JL Thomas, A Eichmann
    Nat Commun, 2019-05-28;10(1):2350.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Foxc2 is essential for podocyte function
    Authors: D Nilsson, M Heglind, Z Arani, S Enerbäck
    Physiol Rep, 2019-05-01;7(9):e14083.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability
    Authors: M Riddell, A Nakayama, T Hikita, F Mirzapours, T Kawamura, A Pasha, M Li, M Masuzawa, M Looso, T Steinbache, K Ebnet, M Potente, T Hirose, S Ohno, I Fleming, S Gattenlöhn, PP Aung, T Phung, O Yamasaki, T Yanagi, H Umemura, M Nakayama
    Nat Commun, 2018-12-17;9(1):5357.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Mice doubly deficient in Six4 and Six5 show ventral body wall defects reproducing human omphalocele
    Authors: M Takahashi, M Tamura, S Sato, K Kawakami
    Dis Model Mech, 2018-10-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer
    Authors: M Ouarné, C Bouvard, G Boneva, C Mallet, J Ribeiro, A Desroches-, E Soleilhac, E Tillet, O Peyruchaud, S Bailly
    J. Exp. Clin. Cancer Res., 2018-08-30;37(1):209.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy
    Authors: TJ Chozinski, C Mao, AR Halpern, JW Pippin, SJ Shankland, CE Alpers, B Najafian, JC Vaughan
    Sci Rep, 2018-07-10;8(1):10396.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF
    Authors: E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi
    EMBO Rep., 2018-04-11;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  19. Loss of ezrin expression reduced the susceptibility to the glomerular injury in mice
    Authors: R Hatano, A Takeda, Y Abe, K Kawaguchi, I Kazama, M Matsubara, S Asano
    Sci Rep, 2018-03-14;8(1):4512.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury
    Authors: DO Dias, H Kim, D Holl, B Werne Soln, J Lundeberg, M Carlén, C Göritz, J Frisén
    Cell, 2018-03-01;173(1):153-165.e22.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  21. Pleiotropic activity of systemically delivered angiogenin in the SOD1G93Amouse model
    Authors: M Crivello, SL O'Riordan, I Woods, S Cannon, L Halang, KS Coughlan, MC Hogg, SA Lewandowsk, JHM Prehn
    Neuropharmacology, 2018-02-25;133(0):503-511.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. Loss of mucin-type O-glycans impairs the integrity of the glomerular filtration barrier in the mouse kidney
    Authors: K Song, J Fu, J Song, BH Herzog, K Bergstrom, Y Kondo, JM McDaniel, S McGee, R Silasi-Man, F Lupu, H Chen, H Bagavant, L Xia
    J. Biol. Chem., 2017-08-25;292(40):16491-16497.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  23. Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1
    Authors: SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch
    Sci Rep, 2017-07-07;7(1):4916.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Deletion of Inositol-Requiring Enzyme-1? in Podocytes Disrupts Glomerular Capillary Integrity and Autophagy
    Authors: DR Kaufman, J Papillon, L Larose, T Iwawaki, AV Cybulsky
    Mol. Biol. Cell, 2017-04-20;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice
    Sci Rep, 2016-08-05;6(0):30739.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance
    Nat Commun, 2016-07-29;7(0):12329.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. The development and plasticity of alveolar type 1 cells
    Authors: J Yang, BJ Hernandez, D Martinez A, O Narvaez de, L Vila-Ellis, H Akiyama, SE Evans, EJ Ostrin, J Chen
    Development, 2015-11-19;143(1):54-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  28. Endothelial ALK1 Is a Therapeutic Target to Block Metastatic Dissemination of Breast Cancer.
    Authors: Cunha S, Bocci M, Lovrot J, Eleftheriou N, Roswall P, Cordero E, Lindstrom L, Bartoschek M, Haller B, Pearsall R, Mulivor A, Kumar R, Larsson C, Bergh J, Pietras K
    Cancer Res, 2015-06-15;75(12):2445-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  29. Clustered PI(4,5)P(2) accumulation and ezrin phosphorylation in response to CLIC5A.
    Authors: Al-Momany A, Li L, Alexander R, Ballermann B
    J Cell Sci, 2014-10-24;127(24):5164-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  30. Protein tyrosine phosphatase 1B inhibition protects against podocyte injury and proteinuria.
    Authors: Kumagai T, Baldwin C, Aoudjit L, Nezvitsky L, Robins R, Jiang R, Takano T
    Am J Pathol, 2014-06-18;184(8):2211-24.
    Species: Mouse, Rat
    Sample Types: Whole Tissue
    Applications: ICC
  31. Direct action of endothelin-1 on podocytes promotes diabetic glomerulosclerosis.
    Authors: Lenoir O, Milon M, Virsolvy A, Henique C, Schmitt A, Masse J, Kotelevtsev Y, Yanagisawa M, Webb D, Richard S, Tharaux P
    J Am Soc Nephrol, 2014-04-10;25(5):1050-62.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  32. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014-03-12;507(7492):376-80.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  33. Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling.
    Authors: Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R
    Development, 2013-12-18;141(2):448-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  34. Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) lambda/iota.
    Authors: Worthmann K, Leitges M, Teng B, Sestu M, Tossidou I, Samson T, Haller H, Huber T, Schiffer M
    Am J Pathol, 2013-10-03;183(6):1945-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  35. Deficiency for endoglin in tumor vasculature weakens the endothelial barrier to metastatic dissemination.
    Authors: Anderberg C, Cunha S, Zhai Z, Cortez E, Pardali E, Johnson J, Franco M, Paez-Ribes M, Cordiner R, Fuxe J, Johansson B, Goumans M, Casanovas O, ten Dijke P, Arthur H, Pietras K
    J Exp Med, 2013-02-11;210(3):563-79.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  36. Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.
    Authors: Pierchala BA, Munoz MR, Tsui CC
    Kidney Int., 2010-07-21;78(9):868-82.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  37. Novel regulators of kidney development from the tips of the ureteric bud.
    Authors: Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J
    J. Am. Soc. Nephrol., 2005-05-25;16(7):1993-2002.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Mouse Podocalyxin Antibody

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Mouse Podocalyxin Antibody
By Anonymous on 05/04/2021
Application: ICC/IF Sample Tested: Adult brain Species: Mouse

Dilution 1:200

Immunocytochemistry/Immunofluorescence Mouse Podocalyxin Antibody AF1556
Mouse Podocalyxin Antibody
By Anonymous on 02/20/2017
Application: IHC Sample Tested: Adult heart Species: Mouse

10um think hearts sections. Fixed with 4% PFA at RT for 10mins, permeabilized using 0.3% triton for 30mins at RT followed by 1% BSA block. Incubated with Goat Podocalyxin (1/100 concentration) at 4’C – O/N. Washed using 1xPBS, Detected using Donkey anti-goat alexa 568 (1/200 concentration). Washed and mounted using prolong antifade reagent with DAPI

Immunohistochemistry Mouse Podocalyxin Antibody AF1556
Mouse Podocalyxin Antibody
By Stephen Wilson on 04/22/2016
Sample Tested: Tracheal whole mount Species: Mouse

Immunofluorescence staining of whole mount tracheal blood vessels. 1:200 dilution

Mouse Podocalyxin Antibody AF1556