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Mouse Podocalyxin Biotinylated Antibody

Catalog #: BAF1556
4 Citations Datasheet / COA / SDS
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BAF1556
Product Details
Citations (4)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Podocalyxin Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Podocalyxin in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
His22-Arg402
Accession # Q9R0M4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin
Purity
Antigen Affinity-purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Podocalyxin
Flow Cytometry
2.5 µg/106 cells
D3 mouse embryonic stem cell line
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse liver and thymus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References
  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.
Entrez Gene IDs
5420 (Human); 27205 (Mouse)
Alternate Names
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

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Citations for Mouse Podocalyxin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. PGC1alpha is required for the renoprotective effect of lncRNA Tug1 in�vivo and links Tug1 with urea cycle metabolites
    Authors: L Li, J Long, K Mise, DL Galvan, PA Overbeek, L Tan, SV Kumar, WK Chan, PL Lorenzi, BH Chang, FR Danesh
    Cell Reports, 2021-08-10;36(6):109510.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1
    Authors: J Long, DL Galvan, K Mise, YS Kanwar, L Li, N Poungvarin, PA Overbeek, BH Chang, FR Danesh
    J. Biol. Chem., 2020-05-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Isolation
  3. CNTO 530 functions as a potent EPO mimetic via unique sustained effects on bone marrow proerythroblast pools.
    Authors: Sathyanarayana P, Houde E, Marshall D, Volk A, Makropoulos D, Emerson C, Pradeep A, Bugelski PJ, Wojchowski DM
    Blood, 2009-03-05;113(20):4955-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Erythropoietin modulation of podocalyxin and a proposed erythroblast niche.
    Authors: Sathyanarayana P, Menon MP, Bogacheva O, Bogachev O, Niss K, Kapelle WS, Houde E, Fang J, Wojchowski DM
    Blood, 2007-04-02;110(2):509-18.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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