Mouse Podocalyxin PE-conjugated Antibody

Catalog #: FAB1556P
1 Citation Datasheet / COA / SDS

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FAB1556P

Detection of Podocalyxin in bEnd.3 Mouse Cell Line by Flow Cytometry.

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All Podocalyxin Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Podocalyxin PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Podocalyxin in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2B Clone # 192703

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
Ser21-Arg402
Accession # Q9R0M4

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Podocalyxin in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse brain endothelial cell line was stained with Rat Anti-Mouse Podocalyxin PE-conjugated Monoclonal Antibody (Catalog # FAB1556P, filled histogram) or isotype control antibody (IC013P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry

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Detection of Podocalyxin in Undifferentiated D3 Mouse Cell Line by Flow Cytometry. Undifferentiated D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse Podocalyxin PE-conjugated Monoclonal Antibody (Catalog # FAB1556P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References

Li, J. et al. (2001) DNA Seq. 12(5):407.
Hara, T. et al. (1999) Immunity 11(5):567.

Entrez Gene IDs

5420 (Human); 27205 (Mouse)

Alternate Names

GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Mouse Podocalyxin PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Urinary podocyte microparticles identify prealbuminuric diabetic glomerular injury.
Authors: Burger D, Thibodeau J, Holterman C, Burns K, Touyz R, Kennedy C
J Am Soc Nephrol, 2014-03-27;25(7):1401-7.
Species: Mouse
Sample Types: Urine
Applications: Flow Cytometry