Mouse/Rat/Bovine GABA-A R alpha 1 N-Terminus Antibody

Catalog #: PPS023 Datasheet / COA / SDS

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PPS023

Detection of GABAAR alpha 1 N-Terminus by Western Blot

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Mouse/Rat/Bovine GABA-A R alpha 1 N-Terminus Antibody Summary

Species Reactivity

Mouse, Rat, Bovine

Specificity

Mouse and rat ~51 kDa GABA_A R alpha 1 subunit, N-Terminus

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

N-terminus peptide of GABA_A R alpha 1 subunit conjugated to keyhole limpet hemocyanin (KLH)

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of GABA_AR alpha 1 N-Terminus by Western Blot Western blot of forebrain lysates from wildtype and alpha 1 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABA_AR, alpha 1 subunit, N-Terminus diluted 1:1000. The antibody labeled the ~51 kDa alpha 1 subunit of the GABA_AR (Control). The labeling was absent from a lysate prepared from alpha 1 knockout animals.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 1

GABA_A ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABA_A receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABA_A receptors have some combination of alpha, beta, and gamma subunits. The rat alpha 1 isoform is a 50 - 52 kDa, 428 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 15 - 222). As with many receptors, phosphorylation is used as a regulatory mechanism. CaM kinase II is known to phosphorylate the alpha 1 subunit and regulate benzodiazepine binding. alpha 1 subunits are particularly abundant in the cerebellum and may contribute to GABA receptor distribution. In the hippocampus and amygdala, the alpha 1 subunit may contribute to amnesia.

References

Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
Churn, S.B. et al. (2002) J. Neurochem. 82:1065.
Kralic, J.E. et al. (2006) J. Comp. Neurol. 495:408.
Sonner, J.M. et al. (2006) Mol. Pharmacol. 68:61.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-1 Polypeptide

Entrez Gene IDs

2554 (Human); 14394 (Mouse); 29705 (Rat); 282235 (Bovine)

Alternate Names

ECA4; EJM; EJM5GABA(A) receptor subunit alpha-1; GABA A R alpha 1; GABAAR alpha 1; GABAARa1; GABRA1; gamma-aminobutyric acid (GABA) A receptor, alpha 1; gamma-aminobutyric acid receptor subunit alpha-1