Mouse/Rat Cathepsin C/DPPI Antibody Summary
Asp25-Leu462
Accession # P97821
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Cathepsin C/DPPI by Western Blot. Western blot shows lysates of mouse liver tissue and mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cathepsin C/DPPI at approximately 20-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse and Rat Cathepsin C/DPPI by Western Blot. Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cathepsin C/DPPI at approximately 20-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Cathepsin C/DPPI in Mouse Thymus. Cathepsin C/DPPI was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Cathepsin C/ DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to thymocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of Mouse Cathepsin C/DPPI by Simple WesternTM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin C/DPPI at approximately 35 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin C/DPPI
Cathepsin C is a cysteine protease of the papain family (1). Cathepsin C sequentially removes dipeptides from the free N-termini of proteins and peptides. It has broad specificity except that it does not cleave a basic amino acid (Arg or Lys) in the N-terminal position or Pro on either side of the scissle bond. It requires halide ions for activity. The pro form contains a pro peptide and a catalytic region, which can be further processed into heavy/ alpha and light/ beta chains that are linked by a disulfide bond. It is broadly distributed. Cathepsin C plays a role in the lysosomal degradation. It also functions as a key enzyme in the activation of granule serine proteases in cytotoxic T lymphocytes and natural killer cells (granzymes A and B), mast cells (tryptase and chymase), and neutrophils (Cathepsin G and elastase) by removing their N-terminal activation dipeptides (2).
- Turk, B. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, et al. eds. p. 1192, Academic Press, San Diego.
- Dahl, S.W. et al. (2001) Biochemistry 40:1671.
Product Datasheets
Citations for Mouse/Rat Cathepsin C/DPPI Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Inflammatory Serine Proteases Play a Critical Role in the Early Pathogenesis of Diabetic Cardiomyopathy
Authors: MA Kolpakov, K Sikder, A Sarkar, S Chaki, SK Shukla, X Guo, Z Qi, C Barbery, A Sabri, K Rafiq
Cell. Physiol. Biochem., 2019-01-01;53(6):982-998.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
The balance between cathepsin C and cystatin F controls remyelination in the brain of Plp1-overexpressing mouse, a chronic demyelinating disease model
Authors: T Shimizu, W Wisessmith, J Li, M Abe, K Sakimura, B Chetsawang, Y Sahara, K Tohyama, KF Tanaka, K Ikenaka
Glia, 2017-03-02;0(0):.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K
PLoS ONE, 2015-04-30;10(4):e0120348.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells.
Authors: Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T
Mol Cancer, 2015-02-15;14(0):39.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The endolysosomal cysteine cathepsins L and K are involved in macrophage-mediated clearance of Staphylococcus aureus and the concomitant cytokine induction.
Authors: Muller S, Faulhaber A, Sieber C, Pfeifer D, Hochberg T, Gansz M, Deshmukh S, Dauth S, Brix K, Saftig P, Peters C, Henneke P, Reinheckel T
FASEB J, 2013-09-13;28(1):162-75.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Up-regulation of microglial cathepsin C expression and activity in lipopolysaccharide-induced neuroinflammation.
Authors: Fan K, Wu X, Fan B, Li N, Lin Y, Yao Y, Ma J
J Neuroinflammation, 2012-05-20;9(1):96.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
Authors: Shree T, Olson OC, Elie BT
Genes Dev., 2011-12-01;25(23):2465-79.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Gene targeting of the cysteine peptidase cathepsin H impairs lung surfactant in mice.
Authors: Buhling F, Kouadio M, Chwieralski CE, Kern U, Hohlfeld JM, Klemm N, Friedrichs N, Roth W, Deussing JM, Peters C, Reinheckel T
PLoS ONE, 2011-10-12;6(10):e26247.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of {alpha}1-antitrypsin.
Authors: Zhang B, Zheng C, Zhu M, Tao J, Vasievich MP, Baines A, Kim J, Schekman R, Kaufman RJ, Ginsburg D
Blood, 2011-07-27;118(12):3384-91.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
A role for serglycin proteoglycan in mast cell apoptosis induced by a secretory granule-mediated pathway.
Authors: Melo FR, Waern I, Ronnberg E, Abrink M, Lee DM, Schlenner SM, Feyerabend TB, Rodewald HR, Turk B, Wernersson S, Pejler G
J. Biol. Chem., 2010-12-01;286(7):5423-33.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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