Mouse/Rat CNTF Antibody

Catalog #: AF-557-NA
12 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-557-NA
AF-557-SP

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All CNTF Antibodies

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Citations (12)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat CNTF Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects rat CNTF in direct ELISAs and Western blots. Detects mouse and rat CNTF in immunohistochemistry. In direct ELISAs and Western blots, less than 15% cross-reactivity with recombinant human CNTF is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat CNTF
Ala2-Met200
Accession # P20294.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize CNTF-induced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND₅₀) is typically 0.3-2.4 µg/mL in the presence of 20 ng/mL Recombinant Rat CNTF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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CNTF in Mouse Cerebellum. CNTF was detected in perfusion fixed frozen sections of mouse cerebellum using Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

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CNTF in Rat Cerebellum. CNTF was detected in perfusion fixed frozen sections of rat cerebellum using Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

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CNTF in Mouse Brain. CNTF was detected in perfusion fixed paraffin-embedded sections of mouse brain (hippocampus) using Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm of Purkinje cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization

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Cell Proliferation Induced by CNTF and Neutralization by Rat CNTF Antibody. Recom-binant Rat CNTF (Catalog # 557-NT) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA). The ND₅₀ is typ-ically 0.3-2.4 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Long Name

Ciliary Neurotrophic Factor

Entrez Gene IDs

1270 (Human); 12803 (Mouse); 25707 (Rat)

Alternate Names

ciliary neurotrophic factor; CNTF; HCNTF

Product Datasheets

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COA

SDS

Related Research Areas

Citations for Mouse/Rat CNTF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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LZK-dependent stimulation of astrocyte reactivity promotes corticospinal axon sprouting
Authors: Meifan Chen, Laura Ingle, Erik J. Plautz, Xiangmei Kong, Rui Tang, Neil Ghosh et al.
Frontiers in Cellular Neuroscience
Action of Administered Ciliary Neurotrophic Factor on the Mouse Dorsal Vagal Complex
Authors: Martina Senzacqua, Ilenia Severi, Jessica Perugini, Samantha Acciarini, Saverio Cinti, Antonio Giordano
Frontiers in Neuroscience
The astrocytic TRPA1 channel mediates an intrinsic protective response to vascular cognitive impairment via LIF production
Authors: Kakae, M;Nakajima, H;Tobori, S;Kawashita, A;Miyanohara, J;Morishima, M;Nagayasu, K;Nakagawa, T;Shigetomi, E;Koizumi, S;Mori, Y;Kaneko, S;Shirakawa, H;
Science advances
Species: Mouse, Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC
The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis
Authors: P Gómez-Fern, A Lopez de L, I Astobiza, J Mena, A Urtasun, V Altmann, F Matesanz, D Otaegui, E Urcelay, A Antigüedad, S Malhotra, X Montalban, T Castillo-T, L Espino-Pai, O Aktas, M Buttmann, A Chan, B Fontaine, PA Gourraud, M Hecker, S Hoffjan, C Kubisch, T Kümpfel, F Luessi, UK Zettl, F Zipp, I Alloza, M Comabella, CM Lill, K Vandenbroe
Cells, 2020-01-10;9(1):.
Species: Mouse
Sample Types: Cell Lysate
Applications: Western Blot
Therapeutic Potential of AAV1-Rheb(S16H) Transduction Against Alzheimer's Disease
Authors: GJ Moon, S Kim, MT Jeon, KJ Lee, IS Jang, M Nakamura, SR Kim
J Clin Med, 2019-11-22;8(12):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus.
Authors: Severi I, Perugini J, Mondini E, Smorlesi A, Frontini A, Cinti S, Giordano A
Front Neurosci, 2013-12-27;7(0):263.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
AAV-mediated expression of CNTF promotes long-term survival and regeneration of adult rat retinal ganglion cells.
Authors: Leaver SG, Cui Q, Plant GW, Arulpragasam A, Hisheh S, Verhaagen J, Harvey AR
Gene Ther., 2006-05-18;13(18):1328-41.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system.
Authors: Gard AL, Gavin E, Solodushko V, Pennica D
Neuroscience, 2004-01-01;127(1):43-52.
Species: Mouse, Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
Inhibition of the Jak-STAT pathway prevents CNTF-mediated survival of axotomized oxytocinergic magnocellular neurons in organotypic cultures of the rat supraoptic nucleus
Authors: Jason M. Askvig, David Y. Lo, Adam W. Sudbeck, Kathryn E. Behm, Laura J. Leiphon, John A. Watt
Experimental Neurology
Restoration of skilled locomotion by sprouting corticospinal axons induced by co-deletion of PTEN and SOCS3
Authors: Duo Jin, Yuanyuan Liu, Fang Sun, Xuhua Wang, Xuefeng Liu, Zhigang He
Nature Communications

Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress
Authors: Alán Alpár, Péter Zahola, János Hanics, Zsófia Hevesi, Solomiia Korchynska, Marco Benevento et al.
The EMBO Journal

Neuronal activity and axonal sprouting differentially regulate CNTF and CNTF receptor complex in the rat supraoptic nucleus
Authors: Jason M. Askvig, Laura J. Leiphon, John A. Watt
Experimental Neurology