Mouse/Rat Integrin alpha 8 Antibody Summary
Phe38-Phe1007
Accession # A28ARA8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Mouse Integrin alpha 8 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Integrin alpha 8 in 4T1 Mouse Breast Cancer Cell Line. Integrin a8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
View Larger
Detection of Mouse and Rat Integrin alpha 8 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 8
Integrin alpha 8 is a 170‑200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. alpha 8 beta 1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse Integrin alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 969 aa extracellular region (aa 38‑1006) and a 29 aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin alpha 8 shares 96% and 90% aa sequence identity with rat and human Integrin alpha 8 protein, respectively.
Product Datasheets
Citations for Mouse/Rat Integrin alpha 8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Measurement of adhesion and traction of cells at high yield (MATCHY) reveals an energetic ratchet driving nephron condensation
Authors: Liu, J;Prahl, LS;Huang, A;Hughes, AJ;
bioRxiv : the preprint server for biology
Species: Murine polyomavirus strain A3
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Progenitor-derived endothelin controls dermal sheath contraction for hair follicle regression
Authors: P Martino, R Sunkara, N Heitman, M Rangl, A Brown, N Saxena, L Grisanti, D Kohan, M Yanagisawa, M Rendl
Nature Cell Biology, 2023-01-30;0(0):.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing
Authors: S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie
Cell Stem Cell, 2020-08-04;27(3):396-412.e6.
Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Aberrant DNA methylation of Tgfb1 in diabetic kidney mesangial cells
Authors: S Oba, N Ayuzawa, M Nishimoto, W Kawarazaki, K Ueda, D Hirohama, F Kawakami-M, T Shimosawa, T Marumo, T Fujita
Sci Rep, 2018-11-05;8(1):16338.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Role of integrin alpha8 in murine model of lung fibrosis
Authors: CF Hung, CL Wilson, YH Chow, LM Schnapp
PLoS ONE, 2018-05-29;13(5):e0197937.
Species: Mouse
Sample Types: Protein, Whole Tissue
Applications: IHC, Western Blot -
Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development
Authors: N Naiman, K Fujioka, M Fujino, MT Valerius, SS Potter, AP McMahon, A Kobayashi
Dev. Cell, 2017-05-22;41(4):349-365.e3.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Activation of Hypoxia Signaling in Stromal Progenitors Impairs Kidney Development
Authors: K Gerl, D Steppan, M Fuchs, C Wagner, C Willam, A Kurtz, B Kurt
Am. J. Pathol., 2017-05-17;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
J. Immunol, 2017-02-20;0(0):.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC -
X-Linked Alport Dogs Demonstrate Mesangial Filopodial Invasion of the Capillary Tuft as an Early Event in Glomerular Damage
PLoS ONE, 2016-12-13;11(12):e0168343.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC -
Laminin alpha2-mediated focal adhesion kinase activation triggers Alport glomerular pathogenesis.
Authors: Delimont D, Dufek B, Meehan D, Zallocchi M, Gratton M, Phillips G, Cosgrove D
PLoS ONE, 2014-06-10;9(6):e99083.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
Nature, 2013-12-12;504(7479):277-81.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Basement membrane assembly of the integrin alpha8beta1 ligand nephronectin requires Fraser syndrome-associated proteins.
J. Cell Biol., 2012-05-21;197(5):677-89.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse/Rat Integrin alpha 8 Antibody
Average Rating: 5 (Based on 3 Reviews)
Have you used Mouse/Rat Integrin alpha 8 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Stables HEK293 expressing ITGA8 were lysed in RIPA and used for western blot analysis. Blot was incubated overnight with 5% milk blocking solution followed by an overnight incubation with 0.4ug/ml of goat anti-ITGA8 in 5% milk. Blot was incubated the next day with 1:3,000 anti-goat HRP secondary antibody and developed employing a film processor. 293 ITGA8: Stable cell line. 293: untransfected cells. Actin was used as loading control.
