Mouse/Rat Integrin beta 1/CD29 Antibody

Catalog #: AF2405
11 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2405
AF2405-SP

Detection of Mouse and Rat Integrin beta 1/CD29 by Western Blot.

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Product Details

Citations (11)

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Mouse/Rat Integrin beta 1/CD29 Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse Integrin beta 1/CD29 in direct ELISAs. Detects mosue and rat Integrin beta 1/CD29 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Integrin beta 1 and rhIntegrin beta 3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 4, and rmIntegrin beta 6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1
Gln21-Ala738
Accession # P09055

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

B16‑F10 mouse melanoma cell line

Immunohistochemistry

5-15 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse and Rat Integrin beta 1/CD29 by Western Blot. Western blot shows lysates of L-929 mouse fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PC-3 human prostate cancer cell line, Rat-2 rat embryonic fibroblast cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin beta 1/CD29 at approximately 110-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem. Orexin B, p38a, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse/Rat Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western

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Detection of Mouse and Rat Integrin beta 1/CD29 by Simple Western^TM. Simple Western lane view shows lysates of L-929 mouse fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PC-12 rat adrenal pheochromocytoma cell line, and Rat-2 rat embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin beta 1/CD29 at approximately 153-174 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.

Entrez Gene IDs

3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)

Alternate Names

CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse/Rat Integrin beta 1/CD29 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Tissue Inhibitor of Metalloproteinases-1 Overexpression Mediates Chemoresistance in Triple-Negative Breast Cancer Cells
Authors: Agnello, L;d'Argenio, A;Caliendo, A;Nilo, R;Zannetti, A;Fedele, M;Camorani, S;Cerchia, L;
Cells
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
Mechanical tension mobilizes Lgr6+ epidermal stem cells to drive skin growth
Authors: Y Xue, C Lyu, A Taylor, A Van Ee, A Kiemen, Y Choi, N Khavanian, D Henn, C Lee, L Hwang, E Wier, S Wang, S Lee, A Li, C Kirby, G Wang, PH Wu, D Wirtz, LA Garza, SK Reddy
Science Advances, 2022-04-27;8(17):eabl8698.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC/IF
Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis
Authors: A Heinrich, B Bhandary, SJ Potter, N Ratner, T DeFalco
Cell Reports, 2021-10-26;37(4):109885.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing
Authors: S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie
Cell Stem Cell, 2020-08-04;27(3):396-412.e6.
Species: Transgenic Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC
Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis
Authors: A Heinrich, SJ Potter, L Guo, N Ratner, T DeFalco
Cell Rep, 2020-04-14;31(2):107513.
Species: Mouse
Sample Types: Testes
Applications: IF
Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
Nat. Cell Biol, 2017-02-20;19(3):189-201.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Exon skipping restores dystrophin expression, but fails to prevent disease progression in later stage dystrophic dko mice.
Authors: Wu, B, Cloer, C, Lu, P, Milazi, S, Shaban, M, Shah, S N, Marston-Poe, L, Moulton, H M, Lu, Q L
Gene Ther, 2014-06-19;21(9):785-93.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
Authors: Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST
J. Neurochem., 2011-01-19;118(4):521-32.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation
Loss of beta1 integrin in mouse fibroblasts results in resistance to skin scleroderma in a mouse model.
Authors: Liu S, Kapoor M, Denton CP, Abraham DJ, Leask A
Arthritis Rheum., 2009-09-01;60(9):2817-21.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Galectin-7 in the control of epidermal homeostasis after injury.
Authors: Gendronneau G, Sidhu SS, Delacour D, Dang T, Calonne C, Houzelstein D, Magnaldo T, Poirier F
Mol. Biol. Cell, 2008-10-01;19(12):5541-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
Authors: Chang Y, Finnemann SC
J. Cell. Sci., 2007-08-07;120(0):3053-63.
Species: Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation