Mouse/Rat P-Selectin/CD62P Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse and Rat P-Selectin/CD62P by Western Blot. Western blot shows lysates of mouse platelets and rat platelets. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse/Rat P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for P-Selectin/CD62P at approximately 140-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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P‑Selectin/CD62P in Mouse Liver.
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Detection of Rat P-Selectin/CD62P by Simple WesternTM. Simple Western lane view shows lysates of rat platelets, loaded at 0.2 mg/mL. A specific band was detected for P-Selectin/CD62P at approximately 197 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: P-Selectin/CD62P
Mouse P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1). P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. Mouse P-Selectin cDNA encodes a 768 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH 2 -terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.
- Kansas, G.S. (1996) Blood 88:3259.
- McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.
Product Datasheets
Citations for Mouse/Rat P-Selectin/CD62P Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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CNS-associated macrophages contribute to intracerebral aneurysm pathophysiology
Authors: Glavan, M;Jelic, A;Levard, D;Frösen, J;Keränen, S;Franx, BAA;Bras, AR;Louet, ER;Dénes, Á;Merlini, M;Vivien, D;Rubio, M;
Acta neuropathologica communications
Species: Murine polyomavirus strain A3
Sample Types: In Vivo
Applications: In vivo assay -
A dhesion analysis via a tumor vasculature-like microfluidic device identifies CD8+ T�cells with enhanced tumor homing to improve cell therapy
Authors: CP Camargo, AK Muhuri, Y Alapan, LF Sestito, M Khosla, MP Manspeaker, AS Smith, CM Paulos, SN Thomas
Cell Reports, 2023-02-26;42(3):112175.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Resistance to tyrosine kinase inhibitors promotes renal cancer progression through MCPIP1 tumor-suppressor downregulation and c-Met activation
Authors: P Marona, J Górka, O Kwapisz, J Jura, J Rys, RM Hoffman, K Miekus
Cell Death & Disease, 2022-09-22;13(9):814.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Disrupting tumour vasculature and recruitment of aPDL1-loaded platelets control tumour metastasis
Authors: H Li, Z Wang, Z Chen, T Ci, G Chen, D Wen, R Li, J Wang, H Meng, R Bryan Bell, Z Gu, G Dotti, Z Gu
Nature Communications, 2021-05-13;12(1):2773.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC -
Angiopoietin-2 blockade ameliorates autoimmune neuroinflammation by inhibiting leukocyte recruitment into the CNS
Authors: Z Li, EA Korhonen, A Merlini, J Strauss, E Wihuri, H Nurmi, S Antila, J Paech, U Deutsch, B Engelhardt, S Chintharla, GY Koh, A Flügel, K Alitalo
J. Clin. Invest., 2020-04-01;0(0):.
Species: Mouse
Sample Types: Tissue
Applications: IHC-P -
Alcohol exposure-induced neurovascular inflammatory priming impacts ischemic stroke and is linked with brain perivascular macrophages
Authors: A Drieu, A Lanquetin, D Levard, M Glavan, F Campos, A Quenault, E Lemarchand, M Naveau, AL Pitel, J Castillo, D Vivien, M Rubio
JCI Insight, 2020-02-27;0(0):.
Species: Mouse
Sample Types: In Vivo, Whole Tissue
Applications: IHC, In Vivo -
Dose-Dependent Influences of Ethanol on Ischemic Stroke: Role of Inflammation
Authors: G Xu, C Li, AL Parsiola, J Li, KD McCarter, R Shi, WG Mayhan, H Sun
Front Cell Neurosci, 2019-02-12;13(0):6.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Prediction of disease activity in models of multiple sclerosis by molecular magnetic resonance imaging of P-selectin
Authors: AP Fournier, A Quenault, S Martinez d, M Gauberti, G Defer, D Vivien, F Docagne, R Macrez
Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Tumour-specific PI3K inhibition via nanoparticle-targeted delivery in head and neck squamous cell carcinoma
Authors: A Mizrachi, Y Shamay, J Shah, S Brook, J Soong, VK Rajasekhar, JL Humm, JH Healey, SN Powell, J Baselga, DA Heller, A Haimovitz-, M Scaltriti
Nat Commun, 2017-02-13;8(0):14292.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Molecular magnetic resonance imaging discloses endothelial activation after transient ischaemic attack
Authors: Aurélien Quenault
Brain, 2016-11-08;140(0):146-157.
Species: Mouse
Sample Types: Complex Sample Type, Whole Tissue
Applications: Functional Assay, IHC -
Reduced plaque formation induced by rosiglitazone in an STZ-diabetes mouse model of atherosclerosis is associated with downregulation of adhesion molecules.
Authors: Tikellis C, Jandeleit-Dahm KA, Sheehy K, Murphy A, Chin-Dusting J, Kling D, Sebokova E, Cooper ME, Mizrahi J, Woollard KJ
Atherosclerosis, 2008-02-21;199(1):55-64.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis.
Authors: Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC, Vlasuk GP, Pittman DD
Atherosclerosis, 2008-02-21;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
A TREM family member, TLT-1, is found exclusively in the alpha-granules of megakaryocytes and platelets.
Authors: Washington AV, Schubert RL, Quigley L, Disipio T, Feltz R, Cho EH, McVicar DW
Blood, 2004-04-20;104(4):1042-7.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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dilution: 1/1000
