Mouse/Rat SODD/BAG4 Antibody

Catalog #: AF4349 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4349
AF4349-SP

Detection of Mouse/Rat SODD/BAG4 by Western Blot.

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All SODD/BAG4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat SODD/BAG4 Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects endogenous mouse and rat SODD/BAG4 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse SODD/BAG4
Met1-Leu457
Accession # Q8CI61

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Knockout Validated

SODD/BAG4 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in SODD/BAG4 knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse/Rat SODD/BAG4 by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line, CH-1 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse/Rat SODD/BAG4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4349) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). Specific bands were detected for SODD/BAG4 at approximately 65 - 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Knockout Validated

Western Blot Shows Human SODD/BAG4 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human SODD/BAG4 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SODD/BAG4 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat SODD/BAG4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4349) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SODD/BAG4 at approximately 72 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SODD/BAG4

Tumor necrosis factor receptor-1 (TNF-R1) and other TNF receptor superfamily members, such as DR3, contain intracellular death domains (DD) and are capable of initiating apoptosis when activated by their ligands. Silencer of Death Domains (SODD) was identified as being involved in the cellular mechanism to protect against ligand-independent signaling by TNF-R1 and other DD receptors. SODD, also known as Bcl-2-Associated Athanogene 4 (BAG4), is a 457 amino acid (aa), anti‑apoptotic protein that functions through interactions with a variety of proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors, and members of the heat shock protein 70 kDa family. SODD is a ubiquitously expressed, cytoplasmic protein that contains a C terminal BAG domain that can bind and inhibit the chaperone activity of Hsc70/Hsp70. The association of SODD with the DD of TNF-R1 prevents constitutive activation of the TNF-R1 signaling pathway. Binding of TNF to TNF-R1 releases SODD and permits adapter molecules such as TRADD to associate with TNF-R1 leading to the activation of TNF signaling pathways such as apoptosis and NF kappa B activation.

Long Name

Silencer of Death Domains

Entrez Gene IDs

9530 (Human); 67384 (Mouse); 361167 (Rat)

Alternate Names

BAG4; BAG-4; BAG-family molecular chaperone regulator-4; Bcl-2-associated athanogene 4; BCL2-associated athanogene 4; Silencer of death domains; SODD; SODDBAG family molecular chaperone regulator 4