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Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody

Catalog #: AF2985
1 Citation Datasheet / COA / SDS
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AF2985
AF2985-SP
Detection of Mouse and Rat TRH-degrading Ectoenzyme/ TRHDE by Western Blot.
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Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat TRH-degrading Ectoenzyme/TRHDE in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TRH-degrading Ectoenzyme/TRHDE
Arg64-His1025
Accession # Q8K093
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat TRH-degrading Ectoenzyme/ TRHDE antibody by Western Blot. View Larger

Detection of Mouse and Rat TRH-degrading Ectoenzyme/ TRHDE by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and embryonic rat brain hippocampal glial. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TRH-degrading Ectoenzyme/TRHDE at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRH-degrading Ectoenzyme/TRHDE

TRHDE, also known as pyroglutamyl peptidase II and thyroliberinase, is a metalloprotease that specifically removes pyroglutamate from thyrotropin-releasing hormone, a tripeptide of L-pyroglutamyl-L-histidyl-L-prolineamide. TRH functions as a hypothalamic hypophysiotropic neuropeptide and neurotransmitter/neuromodulator within the central nervous system (1). Inhibitors of TRHDE have potential applications as research and therapeutic agents because TRHDE inactivates TRH (2). TRHDE is a type II transmembrane protein and a soluble form is also present in the serum (1). The recombinant mouse TRHDE corresponds to the ectodomain of the enzyme. Its amino acid sequence is 97% and 95% identical to that of rat and human.

References
  1. Schmitmeier, S. et al. (2002) Eur. J. Biochem. 269:1278.
  2. Kelly, J.A. et al. (2005) Biochem. J. 389:569.
Long Name
Thyrotropin Releasing Hormone-degrading Ectoenzyme
Entrez Gene IDs
29953 (Human); 237553 (Mouse)
Alternate Names
EC 3.4.19.6; FLJ22381; PAP-II; PAP-IIthyroliberinase; PGPEP2; Pyroglutamyl-peptidase II; Thyroliberinase; thyrotropin-releasing hormone degrading enzyme; thyrotropin-releasing hormone-degrading ectoenzyme; TRHDE; TRH-DE; TRHdegrading Ectoenzyme; TRH-degrading Ectoenzyme; TRH-DEthyrotropin-releasing hormone degrading ectoenzyme; TRH-specific aminopeptidase

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Citation for Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. NMDA receptor up-regulates pyroglutamyl peptidase II activity in the rat hippocampus.
    Authors: Rodriguez-Molina V, Vargas MA, Joseph-Bravo P, Charli JL
    Neurosci. Lett., 2008-11-07;449(3):211-4.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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