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Mouse Reg3B Antibody

Catalog #: AF5110
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5110
AF5110-SP
Reg3B in Mouse Small Intestine.
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Citations (7)
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Mouse Reg3B Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant rat (rr) Reg3B is observed. In Western blots approximately 30% cross-reactivity with rrReg3B is observed. In direct ELISAs and Western blots, less than 1% cross‑reactivity with rrReg3A, recombinant mouse (rm) Reg3A, and rmReg3G is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Reg3B
Glu27-Gly175
Accession # P35230
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Reg3B
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Reg3B antibody in Mouse Small Intestine by Immunohistochemistry (IHC-Fr). View Larger

Reg3B in Mouse Small Intestine. Reg3B was detected in immersion fixed frozen sections of mouse small intestine using 10 µg/mL Sheep Anti-Mouse Reg3B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5110) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg3B

Reg3B (regenerating islet-derived protein III beta; also PAP-I and HIP) is a secreted, 17 kDa member of the Reg gene family, C-type lectin gene superfamily. It is found in pancreatic acinar cells, intestinal enterocytes and developing spinal motoneurons. Reg3B may play a role in intestinal epithelial cell renewal. The mouse Reg3B precursor is 175 amino acids (aa) in length. It contains a 26 aa signal sequence plus a 149 aa mature region that possesses one C-type lectin domain (aa 38‑173). Mature mouse Reg3B is 59%, 71% and 51% aa identical to the products of three other Reg3 named genes termed RegA, G, and D, respectively. Mature mouse Reg3B is also 91% and 67% aa identical to rat and human Reg3B, respectively.

Long Name
Regenerating Islet-derived IIIb
Entrez Gene IDs
18489 (Mouse); 24618 (Rat)
Alternate Names
PAP1; Reg3B

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Citations for Mouse Reg3B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense
    Authors: FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang
    Cellular and Molecular Gastroenterology and Hepatology, 2021-03-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Regenerating islet-derived protein (Reg)3&beta plays a crucial role in attenuation of ileitis and colitis in mice
    Authors: R Shindo, T Katagiri, S Komazawa-S, M Ohmuraya, W Takeda, Y Nakagawa, N Nakagata, T Sakuma, T Yamamoto, C Nishiyama, T Nishina, S Yamazaki, H Kameda, H Nakano
    Biochem Biophys Rep, 2020-02-07;21(0):100738.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. IFN-I and IL-22 mediate protective effects of intestinal viral infection
    Authors: JA Neil, Y Matsuzawa-, E Kernbauer-, SL Schuster, S Sota, M Venzon, S Dallari, A Galvao Net, A Hine, D Hudesman, P Loke, TJ Nice, K Cadwell
    Nat Microbiol, 2019-06-10;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: IHC
  4. Fetal exposure to maternal inflammation does not affect postnatal development of genetically-driven ileitis and colitis.
    Authors: Hemmerling J, Heller K, Hormannsperger G, Bazanella M, Clavel T, Kollias G, Haller D
    PLoS ONE, 2014-05-21;9(5):e98237.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr, IHC-P
  5. GM-CSF produced by nonhematopoietic cells is required for early epithelial cell proliferation and repair of injured colonic mucosa.
    Authors: Egea, Laia, McAllister, Christop, Lakhdari, Omar, Minev, Ivelina, Shenouda, Steve, Kagnoff, Martin F
    J Immunol, 2013-01-16;190(4):1702-13.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Mucin Muc2 deficiency and weaning influences the expression of the innate defense genes Reg3β, Reg3γ and angiogenin-4.
    Authors: Burger-van Paassen N, Loonen LM, Witte-Bouma J, Korteland-van Male AM, de Bruijn AC, van der Sluis M, Lu P, Van Goudoever JB, Wells JM, Dekker J, Van Seuningen I, Renes IB
    PLoS ONE, 2012-06-19;7(6):e38798.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Urinary levels of regenerating islet-derived protein III beta and gelsolin differentiate gentamicin from cisplatin-induced acute kidney injury in rats.
    Authors: Ferreira L, Quiros Y, Sancho-Martinez SM, Garcia-Sanchez O, Raposo C, Lopez-Novoa JM, Gonzalez-Buitrago JM, Lopez-Hernandez FJ
    Kidney Int., 2010-10-27;79(5):518-28.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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