Mouse Sca-1/Ly6 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB1226N Datasheet / COA / SDS

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FAB1226N

Detection of Sca‑1/Ly6 in Mouse Splenocytes by Flow Cytometry.

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Mouse Sca-1/Ly6 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Sca‑1/Ly6 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 177228

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Sca‑1/Ly6 C-terminally truncated Ly-6E allele
Leu27-Gly119
Accession # CAA28351

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Sca‑1/Ly6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE-conjugated Monoclonal Antibody (Catalog # FAB1356P) and either (A) Rat Anti-Mouse Sca-1/Ly6 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1226N) or (B) Rat IgG_2AAlexa Fluor 700 Isotype Control (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Sca-1/Ly6

Stem Cell Antigen-1 (Sca-1) is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

References

Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634.
Spangrude, G.I. et al. (1988) Science 241:58.
Spangrude, G.I. et al. (1993) Blood 82:3327.
Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
Yamamoto, Y. et al. (1996, Blood 88:445.
Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
Welm, B.E. et al. (2002) Dev. Biol. 245:42.
Codias, E.K. et al. (1990) J. Immunol. 144:2197.
Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
Codias, E.K. et al. (1990) J. Immunol. 145:1407.
Flood, P.M. et al. (1990) J. Exp. Med. 172:115.

Long Name

Stem Cell Antigen-1/Lymphocyte Antigen 6

Entrez Gene IDs

110454 (Mouse)

Alternate Names

C6orf23; G6D; Ly6; Ly-6; LY6-D; LY6G6D; lymphocyte antigen 6 family member G6D; MEGT1; NG25; Sca1; Sca-1

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.