Mouse Sca-1/Ly6 Biotinylated Antibody

Catalog #: BAM1226
7 Citations Datasheet / COA / SDS

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BAM1226

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All Sca-1/Ly6 Antibodies

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Citations (7)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Sca-1/Ly6 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Sca-1/Ly6 in Western blots.

Source

Monoclonal Rat IgG_2A Clone # 177228

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E allele
Leu27-Gly119
Accession # CAA28351

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Sca‑1/Ly6
under non-reducing conditions only

Flow Cytometry

2.5 µg/10⁶ cells

Mouse splenocytes

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Sca‑1/Ly6 in Mouse Kidney. Sca-1/Ly6 was detected in perfusion fixed frozen sections of mouse kidney using Mouse Sca-1/Ly6 Biotinylated Monoclonal Antibody (Catalog # BAM1226) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry

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Sca‑1/Ly6 in Mouse Thymus. Sca-1/Ly6 was detected in perfusion fixed frozen sections of mouse thymus using Mouse Sca-1/Ly6 Biotinylated Monoclonal Antibody (Catalog # BAM1226) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sca-1/Ly6

Sca-1 is an 18 kDa phosphatidylositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2,3).Sca-1-positive HSC can be found in the adult bone marrow, fetal liver, and mobilized peripheral blood and spleen in the adult animal (2-7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9-12).

References

Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634.
Spangrude, G.I. et al. (1988) Science 241:58.
Spangrude, G.I. et al. (1993) Blood 82:3327.
Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
Yamamoto, Y. et al. (1996) Blood 88:445.
Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
Welm, B.E. et al. (2002) Dev. Biol. 245:42.
Codias, E.K. et al. (1990) J. Immunol. 144:2197.
Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
Codias, E.K. et al. (1990) J. Immunol. 145:1407.

Long Name

Stem Cell Antigen-1/Lymphocyte Antigen 6

Entrez Gene IDs

110454 (Mouse)

Alternate Names

C6orf23; G6D; Ly6; Ly-6; LY6-D; LY6G6D; lymphocyte antigen 6 family member G6D; MEGT1; NG25; Sca1; Sca-1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse Sca-1/Ly6 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate
Authors: Hansen, SL;Larsen, HL;Pikkupeura, LM;Maciag, G;Guiu, J;Müller, I;Clement, DL;Mueller, C;Johansen, JV;Helin, K;Lerdrup, M;Jensen, KB;
Science advances
Species: Mouse
Sample Types: Organoids
Applications: IHC
Immuno-localization of definitive hematopoietic stem cells in the vascular niche of mouse fetal liver
Authors: Atreyi Biswas, Shailendra Kumar Singh, Gayathri M. Kartha, Satish Khurana
STAR Protocols
SCA-1/Ly6A Mesodermal Skeletal Progenitor Subpopulations Reveal Differential Commitment of Early Limb Bud Cells
Authors: Jessica Cristina Marín-Llera, Carlos Ignacio Lorda-Diez, Juan Mario Hurle, Jesús Chimal-Monroy
Frontiers in Cell and Developmental Biology
Oncogenic BRAF, unrestrained by TGF&beta-receptor signalling, drives right-sided colonic tumorigenesis
Authors: JDG Leach, N Vlahov, P Tsantoulis, RA Ridgway, DJ Flanagan, K Gilroy, N Sphyris, EG Vázquez, DF Vincent, WJ Faller, MC Hodder, A Raven, S Fey, AK Najumudeen, D Strathdee, C Nixon, M Hughes, W Clark, R Shaw, S:CORT con, SR van Hooff, DJ Huels, JP Medema, ST Barry, MC Frame, A Unciti-Bro, SJ Leedham, GJ Inman, R Jackstadt, BJ Thompson, AD Campbell, S Tejpar, OJ Sansom
Nature Communications, 2021-06-08;12(1):3464.
Species: Mouse
Sample Types: Organoid
Applications: IHC
Characterization of radioresistant epithelial stem cell heterogeneity in the damaged mouse intestine
Authors: T Sato, M Sase, S Ishikawa, M Kajita, J Asano, T Sato, Y Mori, T Ohteki
Sci Rep, 2020-05-22;10(1):8308.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration
Authors: S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen
Cell Stem Cell, 2017-12-14;22(1):35-49.e7.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Allergen exposure-induced differences in CD34+ cell phenotype: relationship to eosinophilopoietic responses in different compartments.
Authors: Sergejeva S, Johansson AK, Malmhall C, Lotvall J
Blood, 2003-09-25;103(4):1270-7.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC