Mouse Serpin B8/Proteinase Inhibitor 8 Antibody

Catalog #: AF3588 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3588
AF3588-SP

All Serpin B8/Proteinase Inhibitor 8 Antibodies

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Mouse Serpin B8/Proteinase Inhibitor 8 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serpin B8/Proteinase Inhibitor 8 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Serpin B8, rhSerpin B9, and rhSerpin B6 is observed and less than 1% cross-reactivity with rhSerpin A1, D1, E2, I2, recombinant mouse Serpin A1, C1, E1, F1, and F2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Serpin B8/Proteinase Inhibitor 8
Asp2-Pro374
Accession # O08800

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Serpin B8/Proteinase Inhibitor 8

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Serpin B8/Proteinase Inhibitor 8, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin B8/Proteinase Inhibitor 8

Serpin B8, also known as Proteinase Inhibitor 8 (PI8) and cytoplasmic antiproteinase 2 (CAP2), is a member of the Serpin superfamily of the serine protease inhibitors (1). It inhibits furin, a ubiquitously expressed convertase that processes many pro-proteins (2). Distribution of Serpin B8 alters during kidney regeneration, indicating that it plays a role in inflammation or tissue repair (3).

References

Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
Dahlen, J.R. et al. (1998) J. Biol. Chem. 273:1851.
Gillard, A. (2006) Am. J. Nephrol. 26:34.

Entrez Gene IDs

5271 (Human); 20725 (Mouse); 288937 (Rat)

Alternate Names

CAP2; CAP-2; CAP2clade B (ovalbumin), member 8; Cytoplasmic antiproteinase 2; NK10; Peptidase inhibitor 8; PI8; PI-8; protease inhibitor 8 (ovalbumin type); Serpin B8; serpin peptidase inhibitor, clade B (ovalbumin), member 8