Mouse Serpin E1/PAI-1 Antibody

Catalog #: AF3828
9 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3828
AF3828-SP

Detection of Mouse Serpin E1/PAI‑1 by Western Blot.

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Citations (9)

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Mouse Serpin E1/PAI-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serpin E1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Serpin E1 is observed.

Source

Polyclonal Goat IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Simple Western

12.5 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Serpin E1/PAI‑1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin E1/PAI-1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Mouse Serpin E1/PAI‑1 by Simple Western^TM. Simple Western lane view shows lysates of mouse placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI-1 at approximately 56 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Serpin E1/PAI-1

Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the principal inhibitor of urokinase-type and tissue-type plasminogen activators. It plays a major role in many processes such as angiogenesis, tumor invasion, and obesity. The amino acid sequence of mouse PAI-1 is 89%, 79% and 76% identical to that of rat, human/chimpanzee and dog.

Long Name

Plasminogen Activator Inhibitor

Entrez Gene IDs

5054 (Human); 18787 (Mouse)

Alternate Names

Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse Serpin E1/PAI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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(Pro)renin Receptor Is an Amplifier of Wnt/ beta -Catenin Signaling in Kidney Injury and Fibrosis
Authors: Zhen Li, Lili Zhou, Yongping Wang, Jinhua Miao, Xue Hong, Fan Fan Hou et al.
Journal of the American Society of Nephrology
beta -Arrestin Deficiency Protects Against Pulmonary Fibrosis in Mice and Prevents Fibroblast Invasion of Extracellular Matrix
Authors: Alysia Kern Lovgren, Jeffrey J. Kovacs, Ting Xie, Erin N. Potts, Yuejuan Li, W. Michael Foster et al.
Science Translational Medicine
Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity
Authors: E Duregotti, CM Reumiller, U Mayr, M Hasman, LE Schmidt, SA Burnap, K Theofilato, J Barallobre, A Beran, M Grandoch, A Viviano, M Jahangiri, M Mayr
Nature Communications, 2022-11-25;13(1):7269.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Acquired Deficiency of Peroxisomal Dicarboxylic Acid Catabolism is a Metabolic Vulnerability in Hepatoblastoma
Authors: H Wang, J Lu, X Chen, M Schwalbe, JE Gorka, JA Mandel, J Wang, ES Goetzman, S Ranganatha, SF Dobrowolsk, EV Prochownik
The Journal of Biological Chemistry, 2021-01-13;0(0):100283.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells
Authors: J Miao, J Huang, C Luo, H Ye, X Ling, Q Wu, W Shen, L Zhou
Physiological Reports, 2021-01-01;9(2):e14696.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
C-X-C motif chemokine receptor 4 aggravates renal fibrosis through activating JAK/STAT/GSK3&beta/&beta-catenin pathway
Authors: Y Liu, Q Feng, J Miao, Q Wu, S Zhou, W Shen, Y Feng, FF Hou, Y Liu, L Zhou
J. Cell. Mol. Med., 2020-03-02;0(0):.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot
Wnt/?-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction
Authors: J Miao, J Liu, J Niu, Y Zhang, W Shen, C Luo, Y Liu, C Li, H Li, P Yang, Y Liu, FF Hou, L Zhou
Aging Cell, 2019-07-18;0(0):e13004.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Thymosin beta4 and its degradation product, Ac-SDKP, are novel reparative factors in renal fibrosis.
Authors: Zuo Y, Chun B, Potthoff S, Kazi N, Brolin T, Orhan D, Yang H, Ma L, Kon V, Myohanen T, Rhaleb N, Carretero O, Fogo A
Kidney Int, 2013-06-05;84(6):1166-75.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Exogenous Wnt1 Prevents Acute Kidney Injury and Its Subsequent Progression to Chronic Kidney Disease
Authors: Xue Hong, Yanni Zhou, Dedong Wang, Fuping Lyu, Tianjun Guan, Youhua Liu et al.
Frontiers in Physiology