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Mouse Serum Amyloid A1/A2 Antibody

Catalog #: AF2948
20 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2948
AF2948-SP
Detection of Mouse Serum Amyloid A1/A2 by Western Blot.
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Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Serum Amyloid A1/A2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Serum Amyloid A1/SAA1 and mouse Serum Amyloid A2/SAA2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Serum Amyloid A1
Gly20-Tyr122
Accession # P05366
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Serum Amyloid A DuoSet ELISA Kit (Catalog # DY2948-05) for convenient development of a sandwich ELISA or the Mouse Serum Amyloid A Quantikine ELISA Kit (Catalog # MSAA00) for a complete optimized ELISA.

 
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Serum Amyloid A1/A2 antibody by Western Blot. View Larger

Detection of Mouse Serum Amyloid A1/A2 by Western Blot. Western blot shows mouse serum and mouse plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse Serum Amyloid A1/A2 antibody by Western Blot. View Larger

Detection of Mouse Serum Amyloid A1/A2 by Western Blot. Western blot shows recombinant mouse Serum Amyloid A1 and recombinant mouse Serum Amyloid A2. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Serum Amyloid A1/A2 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

Serum Amyloid A1/A2 in Mouse Liver. Serum Amyloid A1/A2 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse Serum Amyloid A1/A2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Serum Amyloid A1/A2 by Simple WesternTM. Simple Western lane view shows mouse serum, loaded at a 1:100 dilution. A specific band was detected for Serum Amyloid A1/A2 at approximately 14 kDa (as indicated) using 50 µg/mL of (Catalog # AF2948) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

ELISA Mouse Serum Amyloid A1/A2 Antibody in ELISA Standard Curve. View Larger

Mouse Serum Amyloid A1/A2 ELISA Standard Curve. Recombinant Mouse Serum Amyloid A1/A2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serum Amyloid A1/A2

Mouse serum amyloid A protein-1 (SAA1) is a multifunctional apolipoprotein produced by hepatocytes in response to pro-inflammatory cytokines. It is secreted as a 12 kDa, 103 amino acid (aa), nonglycosylated polypeptide and circulates as part of the HDL complex. The SAA1 gene is one of three SAA genes in mouse, and, based on human, is likely to be allelic. The SAA1 gene product differs from the SAA2 gene product by only nine amino acids. In human, circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one to three amino acids being cleaved from either the N- or C-terminus. The same situation may exist in mouse. The amino acid sequence of mature mouse SAA1 is 72%, 72%, 67%, and 74% identical to mature human, rabbit, equine, and hamster SAA1, respectively.

Alternate Names
PIG4;SAA;SAA1;SAA2;Serum amyloid A-1 protein;Serum amyloid A-2 protein;TP53I4; Serum Amyloid A1/A2

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Citations for Mouse Serum Amyloid A1/A2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Probiotic treatment causes sex-specific neuroprotection after traumatic brain injury in mice
    Authors: Holcomb, M;Marshall, A;Flinn, H;Lozano, M;Soriano, S;Gomez-Pinilla, F;Treangen, TJ;Villapol, S;
    Research square
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  2. Fecal Microbiota Transplantation Derived from Alzheimer's Disease Mice Worsens Brain Trauma Outcomes in Wild-Type Controls
    Authors: S Soriano, K Curry, Q Wang, E Chow, TJ Treangen, S Villapol
    International Journal of Molecular Sciences, 2022-04-19;23(9):.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  3. Promoting mechanism of serum amyloid a family expression in mouse intestinal epithelial cells
    Authors: M Wakai, R Hayashi, Y Ueno, K Onishi, T Takasago, T Uchida, H Takigawa, R Yuge, Y Urabe, S Oka, Y Kitadai, S Tanaka
    PLoS ONE, 2022-03-18;17(3):e0264836.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Fractionated Irradiation of Right Thorax Induces Abscopal Damage on Bone Marrow Cells via TNF-alpha and SAA
    Authors: Y Song, S Hu, J Zhang, L Zhu, X Zhao, Q Chen, J Zhang, Y Bai, Y Pan, C Shao
    International Journal of Molecular Sciences, 2021-09-15;22(18):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Serum Amyloid A1/Toll-Like Receptor-4 Axis, an Important Link between Inflammation and Outcome of TBI Patients
    Authors: V Farré-Alin, A Palomino-A, P Narros-Fer, AB Lopez-Rodr, C Decouty-Pe, A Muñoz-Mont, J Zamorano-F, B Mansilla-F, J Giner-Garc, P García-Fei, M Sáez-Alegr, AJ Palpán-Flo, JM Roda-Frade, CS Carabias, JM Rosa, B Civantos-M, S Yus-Teruel, L Gandía, A Lagares, BJ Hernández-, J Egea
    Biomedicines, 2021-05-25;9(6):.
    Species: Mouse
    Sample Types: Cell Lysate, Whole Tissue
    Applications: IHC, Western Blot
  6. Marked Increased Production of Acute Phase Reactants by Skeletal Muscle during Cancer Cachexia
    Authors: IS Massart, G Paulissen, A Loumaye, P Lause, SA Pötgens, MM Thibaut, E Balan, L Deldicque, A Atfi, E Louis, D Gruson, LB Bindels, MA Meuwis, JP Thissen
    Cancers (Basel), 2020-10-31;12(11):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Comparative plasma proteomics in muscle atrophy during cancer-cachexia and disuse: The search for atrokines
    Authors: S Lim, KR Dunlap, ME Rosa-Caldw, WS Haynie, LT Jansen, TA Washington, NP Greene
    Physiol Rep, 2020-10-01;8(19):e14608.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot
  8. Serum amyloid A-containing HDL binds adipocyte-derived versican and macrophage-derived biglycan, reducing its anti-inflammatory properties
    Authors: CY Han, I Kang, M Omer, S Wang, T Wietecha, TN Wight, A Chait
    JCI Insight, 2020-09-24;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  9. Presence of serum amyloid A3 in mouse plasma is dependent on the nature and extent of the inflammatory stimulus
    Authors: A Chait, LJ den Hartig, S Wang, L Goodspeed, I Babenko, WA Altemeier, T Vaisar
    Sci Rep, 2020-06-25;10(1):10397.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Serum amyloid A is a soluble pattern recognition receptor that drives type 2 immunity
    Authors: U Smole, N Gour, J Phelan, G Hofer, C Köhler, B Kratzer, PA Tauber, X Xiao, N Yao, J Dvorak, L Caraballo, L Puerta, S Rosskopf, J Chakir, E Malle, AP Lane, WF Pickl, S Lajoie, M Wills-Karp
    Nat. Immunol., 2020-06-22;21(7):756-765.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. Serum amyloid A exhibits pH dependent antibacterial action and contributes to host defense against Staphylococcus aureus cutaneous infection
    Authors: H Zheng, H Li, J Zhang, H Fan, L Jia, W Ma, S Ma, S Wang, H You, Z Yin, X Li
    J. Biol. Chem., 2019-12-09;0(0):.
    Species: Mouse
    Sample Types: Protein, Whole Tissue
    Applications: IHC-P, Western Blot
  12. Serum Amyloid A Protein as a Potential Biomarker for Severity and Acute Outcome in Traumatic Brain Injury
    Authors: E Wicker, L Benton, K George, W Furlow, S Villapol
    Biomed Res Int, 2019-04-16;2019(0):5967816.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  13. Increased hypothalamic microglial activation after viral-induced pneumococcal lung infection is associated with excess serum amyloid A production
    Authors: H Wang, M Blackall, L Sominsky, SJ Spencer, R Vlahos, M Churchill, S Bozinovski
    J Neuroinflammation, 2018-07-06;15(1):200.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Serum Amyloid A1 is an epithelial pro-restitutive factor
    Authors: BH Hinrichs, JD Matthews, D Siuda, MN O'Leary, AA Wolfarth, BJ Saeedi, A Nusrat, AS Neish
    Am. J. Pathol., 2018-01-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  15. Amyloid deposition in a mouse model humanized at the transthyretin and retinol-binding protein 4 loci
    Authors: X Li, Y Lyu, J Shen, Y Mu, L Qiang, L Liu, K Araki, BP Imbimbo, KI Yamamura, S Jin, Z Li
    Lab. Invest., 2018-01-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Scavenger receptor A1 prevents metastasis of non-small cell lung cancer via suppression of macrophage serum amyloid A1
    Authors: Y Zhang, Y Wei, B Jiang, L Chen, H Bai, X Zhu, X Li, H Zhang, Q Yang, J Ma, Y Xu, J Ben, DC Christiani, Q Chen
    Cancer Res, 2017-02-15;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. Zymosan-mediated inflammation impairs in vivo reverse cholesterol transport.
    Authors: Malik P, Berisha SZ, Santore J
    J. Lipid Res., 2011-02-19;52(5):951-7.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot
  18. Transmission of circulating cell-free AA amyloid oligomers in exosomes vectors via a prion-like mechanism.
    Authors: Tasaki M, Ueda M, Ochiai S, Tanabe Y, Murata S, Misumi Y, Su Y, Sun X, Shinriki S, Jono H, Shono M, Obayashi K, Ando Y
    Biochem. Biophys. Res. Commun., 2010-08-31;400(4):559-62.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  19. Fish oil increases cholesterol storage in white adipose tissue with concomitant decreases in inflammation, hepatic steatosis, and atherosclerosis in mice.
    Authors: Saraswathi V, Gao L, Morrow JD, Chait A, Niswender KD, Hasty AH
    J. Nutr., 2007-07-01;137(7):1776-82.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  20. Role of APAF-1, E-cadherin and peritumoral lymphocytic infiltration in tumour budding in colorectal cancer.
    Authors: Zlobec I, Lugli A, Baker K, Roth S, Minoo P, Hayashi S, Terracciano L, Jass JR
    J. Pathol., 2007-07-01;212(3):260-8.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

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Reviews for Mouse Serum Amyloid A1/A2 Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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Mouse Serum Amyloid A1/A2 Antibody
By Anonymous on 12/24/2021
Application: ICC/IF Sample Tested: Spinal cord Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse Serum Amyloid A1/A2 Antibody AF2948
Mouse Serum Amyloid A1/A2 Antibody
By Anonymous on 10/17/2018
Application: ICC/IF Sample Tested: Brain tissue Species: Mouse
Mouse Serum Amyloid A1 Antibody
By Anonymous on 10/14/2016
Application: WB Sample Tested: Recombinant protein,Serum Species: Mouse

Protein loaded:

Lane 1: SAA1 50ng
Lane 2: SAA2 50ng
Lane 3: SAA1 10ng
Lane 4: SAA2 10ng
Lane 5: 50nl serum, LPS-treated mouse
Lane 6: 10nl serum, LPS-treated mouse

Recombinant proteins were from R and D systems

Prepared in Invitrogen 1x LDS sample buffer plus 1x reducing agent.

Run on 4-12% Bis-tris gel under reducing conditions in MOPS buffer. Transferred using iBlot 20V 7 min
Blocked 1 hour 5% BSA/TBS-T
Incubated overnight 4 C, antibody prepared 1/1000 in 5% BSA/TBS-T
Secondary antibody was anti-goat from Jackson Immuno 1:5000 for 30 minutes.
West Pico Chemiluminescent Reagent
ImageQuant LAS4000 for imaging

*This antibody gives great signal, but predominantly detects SAA2 over SAA1.

Western Blot Mouse Serum Amyloid A1 Antibody AF2948