Mouse SR-AI/MSR Antibody

Catalog #: MAB1797
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1797
MAB1797-SP

All SR-AI/MSR Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse SR-AI/MSR Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse SR‑AI/MSR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human SR-AI is observed.

Source

Monoclonal Rat IgG2b Clone # 268318

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse SR-AI/MSR (Catalog # 1797-MS)

Flow Cytometry

2.5 µg/10⁶ cells

RAW 264.7 mouse monocyte/macrophage cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: SR-AI/MSR

The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share the common ability to bind and internalize modified forms of Low Density Lipoproteins (mLDL) (1‑3). Family members are classified alphabetically. The A class include four proteins: the three subtypes of SR-A (AI, AII, and AIII) that are generated by alternative splicing of the same gene, and a structurally similar protein named MARCO (4). All A class SRs are multidomain trimeric type II membrane proteins. SR-AI has an N-terminal cytoplasmic domain, a transmembrane domain, a spacer domain, an alpha -helical coiled coil, a collagen-like domain and a C-terminal cysteine-rich domain. SR-A is expressed by most tissue macrophages, dendritic cells and Kupffer cells. It is also highly expressed by microglia in neonatal as well as Alzheimer’ Disease brains. SR-AI binds a broad range of polyanionic ligands including modified proteins (e.g. Oxidized, acetylated or maleylated LDL, Advanced glycation end-product proteins), polyribonucleotides (polyguanosine and polyinosine), polysaccharides (dextran sulfate, fucoidan), phospholipids (phosphatidylserine), bacterial products (lipopolysaccharide and lipoteichoic acid) and selected chemical compounds (silica, crocidolite asbestos). The ligand-binding region has been localized to a positively charged region in the carboxyl end of the collagen-like domain. Based on its ligand binding characteristics, SR-AI is implicated in many physiological and pathophysiological functions. Studies using SR-A knockout mouse have also suggested roles of SR-A in atherogenesis, host defense and innate immunity, acquired immune responses, macrophage adhesion, and phagocytosis of apoptotic cells (1‑3).

References

Daugherty, A. et al. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs 2:223.
Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
Elomaa, O. et al. (1995) Cell 80:603.

Long Name

Macrophage Scavenger Receptor Types I and II

Entrez Gene IDs

4481 (Human); 20288 (Mouse); 25073 (Rat)

Alternate Names

CD204 antigen; CD204; Macrophage acetylated LDL receptor I and II; macrophage scavenger receptor 1; macrophage scavenger receptor type III; MSR1; phSR1; phSR2; SCARA1; SCARA1macrophage scavenger receptor types I and II; Scavenger receptor class A member 1; scavenger receptor class A, member 1; SR-A; SRAI; SR-AI

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse SR-AI/MSR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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CD204 suppresses large heat shock protein-facilitated priming of tumor antigen gp100-specific T cells and chaperone vaccine activity against mouse melanoma.
Authors: Qian J, Yi H, Guo C, Yu X, Zuo D, Chen X, Kane JM, Repasky EA, Subjeck JR, Wang XY
J. Immunol., 2011-08-10;187(6):2905-14.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Silencing of either SR-A or CD36 reduces atherosclerosis in hyperlipidaemic mice and reveals reciprocal upregulation of these receptors.
Authors: Makinen PI, Lappalainen JP, Heinonen SE, Leppanen P, Lahteenvuo MT, Aarnio JV, Heikkila J, Turunen MP, Yla-Herttuala S
Cardiovasc. Res., 2010-07-15;88(3):530-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry