Mouse TACI/TNFRSF13B APC-conjugated Antibody

Catalog #: FAB1041A Datasheet / COA / SDS

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FAB1041A

Detection of TACI/TNFRSF13B in Mouse Splenocytes by Flow Cytometry.

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Mouse TACI/TNFRSF13B APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TACI/TNFRSF13B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) TACI/TNFRSF13B or rhBCMA is observed.

Source

Monoclonal Rat IgG_2A Clone # 166010

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TACI/TNFRSF13B
Phe5-Thr129
Accession # Q9ET35

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TACI/TNFRSF13B in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse TACI/TNFRSF13B APC-conjugated Monoclonal Antibody (Catalog # FAB1041A) or (B) Rat IgG_2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TACI/TNFRSF13B

TACI, Transmembrane Activator and CAML-Interactor, is a member of the TNF receptor superfamily and has been designated TNFRSF13B. TACI is a type III membrane protein with an extracellular N-terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine-rich domains. Within the TNFRSF, it shares the highest homology with B cell Maturation Factor (BCMA). TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Mouse TACI is a 249 amino acid (aa) protein consisting of a 129 aa extracellular domain, a 20 aa transmembrane domain, and a 100 aa intracellular domain. Mouse and human TACI share 54% aa identity.

References

Xia, X.Z. et al. (2000) J. Exp. Med. 192:137.
von Bulow, G.U. et al. (1997) Science 278:138.
Gross, J.A. et al. (2000) Nature 404:995.
Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
Yan, M. et al. (2000) Nature Immunol. 1:37.
Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

Long Name

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Entrez Gene IDs

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Alternate Names

CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B