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Mouse TCR gamma/delta Antibody

Catalog #: MAB7297
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7297
MAB7297-SP
Detection of TCR gamma /δ in Mouse Splenocytes by Flow Cytometry.
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Citations (3)
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Mouse TCR gamma/delta Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TCR gamma /δ.
Source
Monoclonal Hamster IgG2, kappa Clone # GL-3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse intraepithelial lymphocytes
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-reported
Spitzer, M. et al. (2015) Science 349: 1259425.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TCR ?/d antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of TCR gamma /δ in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Hamster Anti-Mouse TCR gamma/d Monoclonal Antibody (Catalog # MAB7297) or (B) Normal Hamster IgG Control Antibody followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCR gamma/delta

The gamma δ T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma and CD3δ glycoprotein subunits of the Ig superfamily. T gamma δ cells develop as a minor population in the thymus and migrate mainly to in skin and intestinal epithelial layers. Mouse and rat CD3 gamma and CD3δ are synthesized as 182 and 173 amino acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and 84 aa extracellular domains (ECD), respectively. The germline ECD sequences of CD3 gamma and CD3δ share 71% and 76% aa identity between mouse and rat, respectively, while both proteins and species share 57-62% aa identity with human CD3 gamma and CD3δ. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma δ T cells, were used as the immunogen for the GL-3/5E11 antibody(1).

References
  1. Goodman, T. and L. Lefrancois (1989) J. Exp. Med. 170:2401569.
Entrez Gene IDs
915 (Human); 12500 (Mouse); 25710 (Rat)
Alternate Names
delta polypeptide; T cell receptor gamma locus; T3D; T-cell antigen receptor, gamma polypeptide; T-cell rearranging gene, gamma; T-cell receptor, gamma cluster; TCR gamma/delta; TCRG; TiT3; TRG

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Citations for Mouse TCR gamma/delta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. IL-35 induces N2 phenotype of neutrophils to promote tumor growth
    Authors: JM Zou, J Qin, YC Li, Y Wang, D Li, Y Shu, C Luo, SS Wang, G Chi, F Guo, GM Zhang, ZH Feng
    Oncotarget, 2017-05-16;8(20):33501-33514.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Dynamics of Circulating gammadelta T Cell Activity in an Immunocompetent Mouse Model of High-Grade Glioma.
    Authors: Beck B, Kim H, O'Brien R, Jadus M, Gillespie G, Cloud G, Hoa N, Langford C, Lopez R, Harkins L, Lamb L
    PLoS ONE, 2015-05-08;10(5):e0122387.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL-1alpha/IL-1R1 expression in chronic obstructive pulmonary disease and mechanistic relevance to smoke-induced neutrophilia in mice.
    Authors: Botelho F, Bauer C, Finch D, Nikota J, Zavitz C, Kelly A, Lambert K, Piper S, Foster M, Goldring J, Wedzicha J, Bassett J, Bramson J, Iwakura Y, Sleeman M, Kolbeck R, Coyle A, Humbles A, Stampfli M
    PLoS ONE, 2011-12-06;6(12):e28457.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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