Mouse Testican 3/SPOCK3 Antibody

Catalog #: MAB2346 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2346
MAB2346-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Testican 3/SPOCK3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Testican 3/SPOCK3 in ELISAs. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant human (rh) Testican 1 is observed and no cross-reactivity with rhTestican 2 is observed.

Source

Monoclonal Rat IgG₁ Clone # 330403

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Testican 3/SPOCK3
Val26-Ile436
Accession # Q8BKV0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Mouse Testican 3/SPOCK Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse Testican 3/SPOCK3 Biotinylated Antibody (Catalog # BAF2346)

Standard: Recombinant Mouse Testican 3/SPOCK3 Protein, CF (Catalog # 2346-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Testican 3/SPOCK3

Testican 3 encoded by the SPOCK3 gene is a proteoglycan expressed in brain (1). Human and mouse Testican 3 share 90% amino acid sequence identity, indicating a conserved function (2, 3). Testican 3 contains Ca²⁺-binding domain and the C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. The N-terminal region, which is unique to Testicans, is responsible for the inhibition of Testican 3 towards MMP-14/MT1-MMP activation of MMP-2 (1). The thyropin domain and the follistatin-like domain with a six cysteine Kazal-like motif may inhibit cysteine and serine proteases, respectively (4). A spliced variant designated as N-Tes contains the N-terminal unique region, the follistatin-like domain and the Ca²⁺-binding domain, but lacks the C-terminal thyropin domain and the acidic domain (1).

References

Nakada, M. et al. (2001) Cancer Res. 61:8896.
Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
Okazaki, Y. et al. (2002) Nature 420:563.
Alliel, P.M. et al. (1993) Eur. J. Biochem. 214:347.

Entrez Gene IDs

50859 (Human); 72902 (Mouse)

Alternate Names

CWCV, and Kazal-like domains proteoglycan 3; HSAJ1454; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3; SPOCK3; TES-3; Testican 3; TICN3