Mouse TGF-beta RII Antibody Summary
Ile24-Asp184
Accession # Q62312
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse TGF-beta RII by Western Blot. Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF532) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for TGF-beta RII at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RII
Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70 - 85 kDa), and Type III (250 - 350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.
The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.
Product Datasheets
Citations for Mouse TGF-beta RII Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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TGF? Signaling Dysregulation May Contribute to COL4A1-Related Glaucomatous Optic Nerve Damage
Authors: Mao, M;Kuo, YM;Yu, AK;Labelle-Dumais, C;Ou, Y;Gould, DB;
Investigative ophthalmology & visual science
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Development of syngeneic murine cell lines for use in immunocompetent orthotopic lung cancer models
Authors: K Nolan, G Verzosa, T Cleaver, D Tippimanch, LN DePledge, XJ Wang, C Young, A Le, R Doebele, H Li, SP Malkoski
Cancer Cell Int., 2020-08-28;20(0):417.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Western Blot -
miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGF? signaling
Authors: J Lam, M van den Bo, J Wegrzyn, J Parker, R Ibrahim, K Slowski, L Chang, S Martinez-H, G Condorelli, M Boldin, Y Deng, P Umlandt, M Fuller, A Karsan
Nat Commun, 2018-06-20;9(1):2418.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
miR-17?92 family clusters control iNKT cell ontogenesis via modulation of TGF-? signaling
Proc. Natl. Acad. Sci. U.S.A, 2016-12-05;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
Proc Natl Acad Sci U S A, 2015-02-17;112(9):E957-65.
Species: Mouse
Sample Types: Whole Tissue
Applications: Flow Cytometry -
Intrinsic TGF-beta signaling promotes age-dependent CD8+ T cell polyfunctionality attrition.
Authors: Bhadra R, Moretto M, Castillo J, Petrovas C, Ferrando-Martinez S, Shokal U, Leal M, Koup R, Eleftherianos I, Khan I
J Clin Invest, 2014-04-24;124(6):2441-55.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Augmented cytoplasmic Smad4 induces acceleration of TGF-beta1 signaling in renal tubulointerstitial cells of hereditary nephrotic ICGN mice with chronic renal fibrosis; possible role for myofibroblastic differentiation.
Authors: Goto Y, Manabe N, Uchio-Yamada K, Yamaguchi-Yamada M, Inoue N, Yamamoto Y, Ogura A, Nagano N, Miyamoto H
Cell Tissue Res., 2003-11-13;315(2):209-21.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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