Mouse Thrombospondin-4 Antibody

Catalog #: AF7860
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7860
AF7860-SP

Detection of Mouse Thrombospondin‑4 by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Thrombospondin-4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Thrombospondin-4 in direct ELISAs and Western blots. In direct ELISAs, aproximately 10% cross-reactivity with recombinant human Thrombospondin-4 is observed, and less than 1% cross-reactivity with recombinant mouse Thrombospondin-1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Thrombospondin-4
Gln27-Asn963 (predicted)
Accession # Q9Z1T2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Thrombospondin‑4 by Western Blot. Western blot shows lysates of mouse muscle tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7860) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thrombospondin-4 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Thrombospondin‑4 in Mouse Skeletal Muscle. Thrombospondin-4 was detected in perfusion fixed frozen sections of mouse skeletal muscle using Sheep Anti-Mouse Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7860) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombospondin-4

Mouse Thrombospondin-4 (TSP-4) is a 120-130 kDa adhesive glycoprotein that belongs to the thrombospondin family of proteins. It is 963 amino acids (aa) in length and highly modular in structure, containing one Lamnin G-like domain, four EGF-like domains and eight type 3 TSP domains. It circulates as a disulfide-linked homopentamer, and is up-regulated in the heart in response to ischemic injury and pathology. Mature mouse TSP-4 is 93% aa identical to human TSP4.

Entrez Gene IDs

7060 (Human); 21828 (Mouse)

Alternate Names

THBS4; thrombospondin 4; Thrombospondin4; Thrombospondin-4; TSP4; TSP-4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Thrombospondin-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Fibrinogen induces neural stem cell differentiation into astrocytes in the subventricular zone via BMP signaling
Authors: L Pous, SS Deshpande, S Nath, S Mezey, SC Malik, S Schildge, C Bohrer, K Topp, D Pfeifer, F Fernández-, S Doostkam, DK Galanakis, V Taylor, K Akassoglou, C Schachtrup
Nat Commun, 2020-01-31;11(1):630.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC
Extracellular matrix/integrin signaling promotes resistance to combined inhibition of HER2 and PI3K in HER2+ breast cancer
Authors: AB Hanker, MV Estrada, G Bianchini, PD Moore, J Zhao, F Cheng, L Gianni, JP Koch, DR Tyson, V Sanchez, BN Rexer, M Sanders, Z Zhao, T Stricker, CL Arteaga
Cancer Res., 2017-04-10;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot