Mouse TIM-1/KIM-1/HAVCR Antibody

Name: Mouse TIM-1/KIM-1/HAVCR Antibody
Brand: R&D Systems
SKU: MAB1817
Rating: 5 (1 reviews)

Catalog #: MAB1817
12 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1817-SP
MAB1817-500
MAB1817-100

All TIM-1/KIM-1/HAVCR Antibodies

Product Details

Citations (12)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TIM‑1/KIM-1/HAVCR in ELISAs and Western blots. This antibody does not cross-react with recombinant human (rh) TIM‑1, rmTIM-2, or rmTIM-3.

Source

Monoclonal Rat IgG_2B Clone # 222414

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIM‑1/KIM-1/HAVCR
Tyr22-Thr212
Accession # NP_001160104

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse TIM‑1/KIM‑1/HAVCR (Catalog # 1817-TM)

Mouse TIM-1/KIM-1/HAVCR Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse TIM‑1/KIM‑1/HAVCR Biotinylated Antibody (Catalog # BAF1817)

Standard: Recombinant Mouse TIM-1/KIM-1/HAVCR Protein (Catalog # 1817-TM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also known as KIM-1 or HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39% and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).

References

Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
Feigelstock, D. et al. (1998) J. Virol. 72:6621.
Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.

Long Name

T Cell Immunoglobulin Mucin-1

Entrez Gene IDs

26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)

Alternate Names

CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Dipeptidase-1 governs renal inflammation during ischemia reperfusion injury
Authors: A Lau, JJ Rahn, M Chappellaz, H Chung, H Benediktss, D Bihan, A von Mässen, A Linkermann, CN Jenne, SM Robbins, DL Senger, IA Lewis, J Chun, DA Muruve
Science Advances, 2022-02-02;8(5):eabm0142.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
A combination of monosodium glutamate and high-fat and high-fructose diets increases the risk of kidney injury, gut dysbiosis and host-microbial co-metabolism
Authors: T Pongking, O Haonon, R Dangtakot, S Onsurathum, A Jusakul, K Intuyod, A Sangka, S Anutrakulc, U Cha'on, S Pinlaor, P Pinlaor
PLoS ONE, 2020-04-08;15(4):e0231237.
Species: Hamster
Sample Types: Whole Tissue
Applications: IHC
Protective Effects of Recombinant Human Soluble Thrombomodulin on Lipopolysaccharide-Induced Acute Kidney Injury
Authors: Y Nozaki, J Ri, K Sakai, K Niki, M Funauchi, I Matsumura
Int J Mol Sci, 2020-04-05;21(7):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Dietary sodium modulates nephropathy in Nedd4-2-deficient mice
Authors: JA Manning, SS Shah, TL Henshall, A Nikolic, J Finnie, S Kumar
Cell Death Differ., 2019-12-04;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Renal immune surveillance and dipeptidase-1 contribute to contrast-induced acute kidney injury
Authors: A Lau, H Chung, T Komada, JM Platnich, CF Sandall, SR Choudhury, J Chun, V Naumenko, BG Surewaard, MC Nelson, A Ulke-Lemée, PL Beck, H Benediktss, AM Jevnikar, SL Snelgrove, MJ Hickey, DL Senger, MT James, JA Macdonald, P Kubes, CN Jenne, DA Muruve
J. Clin. Invest., 2018-06-04;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Proximal tubule-derived colony stimulating factor-1 mediates polarization of renal macrophages and dendritic cells, and recovery in acute kidney injury.
Authors: Wang Y, Chang J, Yao B, Niu A, Kelly E, Breeggemann M, Abboud Werner S, Harris R, Zhang M
Kidney Int, 2015-09-30;88(6):1274-1282.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
KIM-1-mediated phagocytosis reduces acute injury to the kidney.
Authors: Yang L, Brooks C, Xiao S, Sabbisetti V, Yeung M, Hsiao L, Ichimura T, Kuchroo V, Bonventre J
J Clin Invest, 2015-03-09;125(4):1620-36.
Species: Mouse
Sample Types: Cell Lysates
Applications: ELISA Development (Capture)
The pathological role of IL-18Ralpha in renal ischemia/reperfusion injury.
Authors: Yano T, Nozaki Y, Kinoshita K, Hino S, Hirooka Y, Niki K, Shimazu H, Kishimoto K, Funauch M, Matsumura I
Lab Invest, 2014-10-20;95(1):78-91.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
HAVCR/KIM-1 activates the IL-6/STAT-3 pathway in clear cell renal cell carcinoma and determines tumor progression and patient outcome.
Authors: Cuadros T, Trilla E, Sarro E, Vila M, Vilardell J, de Torres I, Salcedo M, Lopez-Hellin J, Sanchez A, Ramon y Cajal S, Itarte E, Morote J, Meseguer A
Cancer Res, 2014-01-03;74(5):1416-28.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Endogenous Tim-1 (Kim-1) promotes T-cell responses and cell-mediated injury in experimental crescentic glomerulonephritis.
Authors: Nozaki Y, Nikolic-Paterson DJ, Snelgrove SL
Kidney Int., 2011-12-28;81(9):844-55.
Species: Mouse
Sample Types: Tissue Homogenates, Urine
Applications: ELISA Development, Western Blot

T cell Ig domain and mucin domain 1 engagement on invariant NKT cells in the presence of TCR stimulation enhances IL-4 production but inhibits IFN-gamma production.
Authors: Kim HS, Kim HS, Lee CW, Chung DH
J. Immunol., 2010-03-10;184(8):4095-106.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.
Authors: Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ
Blood, 2007-07-09;110(7):2565-8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot