Mouse TIMP-1 Antibody

Catalog #: MAB980
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB980-SP
MAB980-500
MAB980-100

All TIMP-1 Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TIMP-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TIMP-1 in ELISAs. In sandwich ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) TIMP‑1, rhTIMP‑2, rhTIMP-3, rhTIMP-4, or rrTIMP-1.

Source

Monoclonal Rat IgG_2B Clone # 151620

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIMP-1
Cys25-Arg205
Accession # P12032

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Mouse TIMP-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse TIMP‑1 Biotinylated Antibody (Catalog # BAF980)

Standard: Recombinant Mouse TIMP-1 Protein, CF (Catalog # 980-MT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of homologous proteins that regulate the activity of matrix metalloproteinases (MMPs) (1, 2). There are four known members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4 that have been found to exhibit multiple functions, including inhibition of active MMPs, proMMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Structurally, TIMPs have two domains, an N‑terminal domain and a C‑terminal domain. Each domain consists of three disulfide-bonded loops. TIMP-1 is a glycoprotein produced by a wide range of cell types. Through its N‑terminal domain, TIMP‑1 inhibits active MMPs by forming a non-covalent binary complex with the MMP active site. The C-terminal domain of TIMP-1 interacts with the C-terminal domain of pro‑MMP‑9, which may play a role in regulating pro‑MMP‑9 activation.

References

Murphy, G. and F. Willenbrock (1995) Methods Enzymol. 248:496.
Brew, K. et al. (2000) Biochim. Biophys. Acta 1477:267.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Entrez Gene IDs

7076 (Human); 21857 (Mouse); 116510 (Rat)

Alternate Names

CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse TIMP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Effects of hydrogen peroxide on wound healing in mice in relation to oxidative damage.
Authors: Loo, Alvin En, Wong, Yee Ting, Ho, Rongjian, Wasser, Martin, Du, Tiehua, Ng, Wee Thon, Halliwell, Barry
PLoS ONE, 2012-11-13;7(11):3023.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development