Mouse TLR2 Antibody

Catalog #: AF1530
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1530
AF1530-SP

Detection of Mouse TLR2 by Western Blot.

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All TLR2 Antibodies

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TLR2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TLR2 in direct ELISAs and Western blots. In these formats, approximately 10% cross-reactivity with recombinant human (rh) TLR2 is observed and less than 2% cross-reactivity with recombinant mouse (rm) TLR1, rhTLR3, rmTLR4, and rmTLR6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TLR2
Gln25-Leu590
Accession # Q9QUN7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse TLR2 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1530) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TLR2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR2

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens (1‑4). To date, there are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1, 3). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular nucleic acids) (3). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. Mouse Toll-like receptor-2 (TLR2) is a 97 kDa, 760 amino acid (aa) glycoprotein that contains a 563 aa extracellular region, a 21 aa TM segment, and a 176 aa cytoplasmic domain (5, 6). The extracellular region contains 16 leucine-rich repeats, while the cytoplasmic tail shows one 146 aa TIR domain. The receptor is expressed on a number of cell types including T cells ( alpha beta and gamma δ), monocytes, dendritic cells, neutrophils, B cells, endothelial cells, mast cells, NK cells, macrophages and hepatocytes (1, 4, 5, 7, 8). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6 (1, 3, 4). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers recognize triacylated lipopeptides from a variety of microorganisms. The TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (9). Biglycan is also known to activate TLR2, but the context is unclear (8). Notably, in human, TLR2 also dimerizes with TLR10. But the TLR10 gene in mouse (but not rat) is mutationally inactive, and thus this complex is nonfunctional (10). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 11). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1, 3). The extracellular region of mouse TLR2 is 89%, 67%, 81%, and 65% aa identical to the equivalent region in rat, human, hamster and canine, respectively.

References

Wetzler, L. (2003) Vaccine 21:S2/55.
Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
Dunne, A. and L. O’Neill (2005) FEBS. Lett. 579:3330.
Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
Matsuguchi, T. et al. (2000) Blood 95:1378.
Meng, G. et al. (2005) Immunol. Lett. 98:200.
Flo T. et al. (2001) J. Leukoc. Biol. 69:474.
Schaefer, L. et al. (2005) J. Clin. Invest. 115:2223.
Akira S. (2003) Curr. Opin. Immunol. 15:5.
Hasan, U. et al. (2005) J. Immunol. 174:2942.
Yamamoto M. et al. (2002) Nature 420:324.

Long Name

Toll-like Receptor 2

Entrez Gene IDs

7097 (Human); 24088 (Mouse); 310553 (Rat)

Alternate Names

CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2

Product Datasheets

Product Datasheet

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SDS

Related Research Areas

Citations for Mouse TLR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Mycobacterium vaccae protects against glucocorticoid resistance resulting from combined physical and psychosocial trauma in mice
Authors: D Langgartne, M Amoroso, E Kempter, M Kustermann, J Scheurer, CA Lowry, G Strau beta, SO Reber
Brain, Behavior, and Immunity, 2023-02-01;109(0):221-234.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection
Authors: Q Hou, J Jia, J Lin, L Zhu, S Xie, Q Yu, Y Li
Cell Reports, 2022-09-27;40(13):111416.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Hemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2.
Authors: Li Y, Xiang M, Yuan Y, Xiao G, Zhang J, Jiang Y, Vodovotz Y, Billiar TR, Wilson MA, Fan J
Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009-10-14;297(6):R1670-80.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Minocycline modulates neuroinflammation independently of its antimicrobial activity in staphylococcus aureus-induced brain abscess.
Authors: Kielian T, Esen N, Liu S, Phulwani NK, Syed MM, Phillips N, Nishina K, Cheung AL, Schwartzman JD, Ruhe JJ
Am. J. Pathol., 2007-08-23;171(4):1199-214.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
TLR-2 is upregulated and mobilized to the hepatocyte plasma membrane in the space of Disse and to the Kupffer cells TLR-4 dependently during acute endotoxemia in mice.
Authors: Ojaniemi M, Liljeroos M, Harju K, Sormunen R, Vuolteenaho R, Hallman M
Immunol. Lett., 2005-09-26;102(2):158-68.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: Electron Microscopy, IHC-P, Western Blot