Mouse TNF RII/TNFRSF1B APC-conjugated Antibody

Catalog #: FAB426A
2 Citations Datasheet / COA / SDS

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FAB426A

Detection of TNF RII/TNFRSF1B in L‑929 Mouse Cell Line by Flow Cytometry.

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Mouse TNF RII/TNFRSF1B APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TNF RII/TNFRSF1B in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) TNF RII/TNFRSF1B, recombinant mouse (rm) TNF RI, rhTNF RI, rm4‑1BB, or rmOPG is observed. In Western blots, approximately 25% cross-reactivity with rhDR3 and rhTNF RII is observed and no cross-reactivity with rm4‑1BB, rmCD27, rmCD30, rmCD40, rmFas, rmGITR, rhHVEM, rhNGF R, rmOPG, rmRANK, rmTNF RI, rmTROY, or rmEDAR is observed.

Source

Monoclonal Hamster IgG Clone # TR7554

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse TNF RII/TNFRSF1B
Extracellular domain

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TNF RII/TNFRSF1B in L‑929 Mouse Cell Line by Flow Cytometry. L‑929 mouse fibroblast cell line was stained with Hamster Anti-Mouse TNF RII/TNFRSF1B APC‑conjugated Monoclonal Antibody (Catalog # FAB426A, filled histogram) or Hamster IgG Allophycocyanin Control Antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TNF RII/TNFRSF1B

Two types of soluble TNF receptors have been identified in human serum and urine which can neutralize the biological activities of TNF-alpha and TNF-beta. These binding proteins represent truncated forms of the two types of high-affinity cell surface receptors for TNF (TNFR-p60 Type B and TNFR-p80 Type A). Soluble TNF RII corresponds to TNFR-p80 Type A. In the new TNF superfamily nomenclature, TNF RII is referred to as TNFRSF1B. These apparent soluble forms of the receptors appear to arise as a result of shedding of the extracellular domains of the membrane-bound receptors. Normal concentrations as high as 4 ng/mL are found in the serum of healthy individuals, and even higher levels may be found in some pathological conditions. Although the physiological role of these proteins is not known, it has been speculated that shedding of the soluble receptors in response to TNF release could serve as a mechanism to scavenge the TNF not immediately bound and thus localize the inflammatory response. It is also possible that the pool of TNF bound to soluble receptors could represent a reservoir for the controlled release of TNF.

Long Name

Tumor Necrosis Factor Receptor II

Entrez Gene IDs

7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)

Alternate Names

CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II

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Citations for Mouse TNF RII/TNFRSF1B APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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TNFR2 limits proinflammatory astrocyte functions during EAE induced by pathogenic DR2b-restricted T cells
Authors: I Raphael, F Gomez-Rive, RA Raphael, RR Robinson, S Nalawade, TG Forsthuber
JCI Insight, 2019-12-19;4(24):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Membrane-organizing protein moesin controls Treg differentiation and antitumor immunity via TGF-? signaling
Authors: EA Ansa-Addo, Y Zhang, Y Yang, GS Hussey, BV Howley, M Salem, B Riesenberg, S Sun, DC Rockey, S Karvar, PH Howe, B Liu, Z Li
J. Clin. Invest, 2017-03-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry