Mouse TSLPR Antibody Summary
Ala20-Leu233
Accession # BAA92159
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TSLP R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF546, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
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Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP R Antibody. Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse IL-7 Ra/CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF546). The ND50 is typically 0.2-0.6 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSLPR
TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The TSLP R cDNA encodes a 359 amino acid (aa) residue type 1 membrane protein with a 24 aa residue signal peptide, a 206 aa residue extracellular domain that showed 24% sequence identity with the mouse common gamma receptor, a 23 aa residue transmembrane domain, and a 106 aa residue cytoplasmic domain. The cytoplasmic domain of TSLP R contains a membrane-proximal box1 motif which is known to be important for association with JAKs. An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3, 4).
- Fujio, K. et al. (2000) Blood 95:2204.
- Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
- Park, L.S. et al. (2000) J. Exp. Med. 192:659.
- Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Product Datasheets
Citations for Mouse TSLPR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Type 2 immunity plays an essential role for murine model of allergic contact dermatitis with mixed type 1/type 2 immune response
Authors: J Park, JW Lee, SH Kim, J Oh, WS Roh, SM Kim, CO Park, MG Lee, TG Kim
Journal of dermatological science, 2021-10-06;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
TSLP Protects Corneas From Pseudomonas aeruginosa Infection by Regulating Dendritic Cells and IL-23-IL-17 Pathway
Authors: X Cui, N Gao, R Me, J Xu, FX Yu
Invest. Ophthalmol. Vis. Sci., 2018-08-01;59(10):4228-4237.
Species: Mouse
Sample Types: In Vivo, Tissue Homogenates, Whole Tissue
Applications: IHC, Neutralization, Western Blot -
Immune Characterization of Bone Marrow-Derived Models of Mucosal and Connective Tissue Mast Cells
Authors: S Benedé, E Cody, C Agashe, MC Berin
Allergy Asthma Immunol Res, 2018-05-01;10(3):268-277.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-7R? Expression Regulates Murine Dendritic Cell Sensitivity to Thymic Stromal Lymphopoietin
Authors: L Kummola, Z Ortutay, X Chen, S Caucheteux, S Hämäläinen, S Aittomäki, R Yagi, J Zhu, M Pesu, WE Paul, IS Junttila
J. Immunol., 2017-04-12;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Rearrangement of CRLF2 in B-progenitor- and Down syndrome-associated acute lymphoblastic leukemia.
Authors: Mullighan CG, Collins-Underwood JR, Phillips LA, Loudin MG, Liu W, Zhang J, Ma J, Coustan-Smith E, Harvey RC, Willman CL, Mikhail FM, Meyer J, Carroll AJ, Williams RT, Cheng J, Heerema NA, Basso G, Pession A, Pui CH, Raimondi SC, Hunger SP, Downing JR, Carroll WL, Rabin KR
Nat. Genet., 2009-10-18;41(11):1243-6.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis.
Authors: Osborn MJ, Ryan PL, Kirchhof N, Panoskaltsis-Mortari A, Mortari F, Tudor KS
Blood, 2003-09-25;103(3):843-51.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
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