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Mouse ULBP-1 /MULT-1 Antibody

Catalog #: MAB2588
14 Citations Datasheet / COA / SDS
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MAB2588
MAB2588-SP
Detection of ULBP‑1 in A20 Mouse Cell Line by Flow Cytometry.
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Citations (14)
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Mouse ULBP-1 /MULT-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ULBP‑1 /MULT-1.
Source
Monoclonal Rat IgG2A Clone # 237104
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with mouse ULBP‑1 /MULT-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
A20 mouse B cell lymphoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of ULBP‑1 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse ULBP-1 /MULT-1 Monoclonal Antibody (Catalog # MAB2588, filled histogram) or isotype control antibody (MAB006, open histogram) followed by anti-Rat IgG PE-conjugated secondary antibody (F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ULBP-1

ULBP-1, also known as MULT-1 (mouse UL16-binding protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1‑4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8+ T cells, epidermal gamma δ T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (KD = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).

References
  1. Raulet, D.H. (2003) Nat. Rev. Immunol. 3:781.
  2. Carayannopoulos, L. et al. (2002) J. Immunol. 169:4079.
  3. Diefenbach, A. et al. (2003) Eur. J. Immunol. 33:381.
  4. Krmpotic, A. et al. (2005) J. Exp. Med. 201:211.
  5. Diefenbach, A. et al. (2002) Nat. Immunol. 3:1142.
  6. Ho, E.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6320.
  7. Carayannopoulos, L.N. et al. (2002) Eur. J. Immunol. 32:597.
Long Name
UL16 Binding Protein-1
Entrez Gene IDs
80329 (Human); 77777 (Mouse)
Alternate Names
ALCAN-beta; MULT-1; N2DL1; N2DL-1; NKG2D ligand 1; NKG2DL; NKG2DL1; RAET1I; RAET1Ialcan-beta; Retinoic acid early transcript 1I; UL16 binding protein 1; UL16-binding protein 1; ULBP1; ULBP-1

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Citations for Mouse ULBP-1 /MULT-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Gut microbiota alterations affect glioma growth and innate immune cells involved in tumor immunosurveillance in mice
    Authors: G D'Alessand, F Antonangel, F Marrocco, A Porzia, C Lauro, A Santoni, C Limatola
    Eur. J. Immunol., 2020-03-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Natural killer cells impede the engraftment of cardiomyocytes derived from induced pluripotent stem cells in syngeneic mouse model
    Authors: Y Nakamura, S Miyagawa, S Yoshida, S Sasawatari, T Toyofuku, K Toda, Y Sawa
    Sci Rep, 2019-07-25;9(1):10840.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Regulation of murine NK cell exhaustion through the activation of the DNA damage repair pathway
    Authors: M Alvarez, F Simonetta, J Baker, A Pierini, AS Wenokur, AR Morrison, WJ Murphy, RS Negrin
    JCI Insight, 2019-06-18;5(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. Immunotherapy of Dual-Function Vector with Both Immunostimulatory and B-Cell Lymphoma 2 (Bcl-2)-Silencing Effects on Gastric Carcinoma
    Authors: L Ma, M Han, Z Keyoumu, H Wang, S Keyoumu
    Med. Sci. Monit., 2017-04-25;23(0):1980-1991.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine.
    Authors: Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud V, Anjuere F
    PLoS ONE, 2015-12-02;10(12):e0143224.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
    Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C
    J Invest Dermatol, 2015-01-29;135(5):1311-9.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  7. MULT1E/mIL-12: a novel bifunctional protein for natural killer cell activation.
    Authors: Tietje A, Li J, Yu X, Wei Y
    Gene Ther, 2014-02-27;21(5):468-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development
  8. The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells.
    Authors: Paolino, Magdalen, Choidas, Axel, Wallner, Stephani, Pranjic, Blanka, Uribesalgo, Iris, Loeser, Stefanie, Jamieson, Amanda M, Langdon, Wallace, Ikeda, Fumiyo, Fededa, Juan Pab, Cronin, Shane J, Nitsch, Roberto, Schultz-Fademrecht, Carsten, Eickhoff, Jan, Menninger, Sascha, Unger, Anke, Torka, Robert, Gruber, Thomas, Hinterleitner, Reinhard, Baier, Gottfrie, Wolf, Dominik, Ullrich, Axel, Klebl, Bert M, Penninger, Josef M
    Nature, 2014-02-19;507(7493):508-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. RAE-1 ligands for the NKG2D receptor are regulated by E2F transcription factors, which control cell cycle entry.
    Authors: Jung, Heiyoun, Hsiung, Benjamin, Pestal, Kathleen, Procyk, Emily, Raulet, David H
    J Exp Med, 2012-11-19;209(13):2409-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Involvement of an NKG2D ligand H60c in epidermal dendritic T cell-mediated wound repair.
    Authors: Yoshida S, Mohamed RH, Kajikawa M, Koizumi J, Tanaka M, Fugo K, Otsuka N, Maenaka K, Yagita H, Chiba H, Kasahara M
    J. Immunol., 2012-03-07;188(8):3972-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
    Authors: Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R
    Blood, 2009-02-06;113(24):6120-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Tumor cells expressing a fusion protein of MULT1 and Fas are rejected in vivo by apoptosis and NK cell activation.
    Authors: Kotturi HS, Li J, Branham-O'Connor M, Stickel SL, Yu X, Wagner TE, Wei Y
    Gene Ther., 2008-05-08;15(19):1302-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Blockade of NKG2D on NKT cells prevents hepatitis and the acute immune response to hepatitis B virus.
    Authors: Vilarinho S, Ogasawara K, Nishimura S, Lanier LL, Baron JL
    Proc. Natl. Acad. Sci. U.S.A., 2007-11-08;104(46):18187-92.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Attribution of NKG2DL to the inhibition of early stage allogeneic tumors in mice
    Authors: Li Hua, Mingli Fang, Boqi Dong, Sheng Guo, Cuiyun Cui, Jiwei Liu et al.
    Oncotarget

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