Mouse Uromodulin Antibody Summary
Ser24-Ala618
Accession # Q91X17
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Uromodulin by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5175) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Uromodulin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Uromodulin in Mouse Kidney. Uromodulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5175) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubule epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Uromodulin
Uromodulin (also Tamm-Horsfall glycoprotein or THP) is a 105‑120 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑148), a ZP domain that mediates oligomerization (aa 335‑590), and a cleavable C-terminal propeptide (aa 619‑642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17 aa substitution for aa 601‑642, and a 166 aa substitution for aa 441‑642. Over aa 25‑618, mouse Uromodulin shares 78% and 89% aa identical to human and rat Uromodulin, respectively.
Product Datasheets
Citations for Mouse Uromodulin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Tamm-Horsfall protein augments neutrophil NETosis during urinary tract infection
Authors: Mercado-Evans, V;Chew, C;Serchejian, C;Saltzman, A;Mejia, ME;Zulk, JJ;Cornax, I;Nizet, V;Patras, KA;
bioRxiv : the preprint server for biology
Species: Murine polyomavirus strain A3, Viral
Sample Types: Whole Cells
Applications: Immunocytochemistry -
LC3 lipidation is essential for TFEB activation during the lysosomal damage response to kidney injury
Authors: S Nakamura, S Shigeyama, S Minami, T Shima, S Akayama, T Matsuda, A Esposito, G Napolitano, A Kuma, T Namba-Hama, J Nakamura, K Yamamoto, M Sasai, A Tokumura, M Miyamoto, Y Oe, T Fujita, S Terawaki, A Takahashi, M Hamasaki, M Yamamoto, Y Okada, M Komatsu, T Nagai, Y Takabatake, H Xu, Y Isaka, A Ballabio, T Yoshimori
Nat Cell Biol, 2020-09-28;22(10):1252-1263.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy
Authors: O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy
Physiol Rep, 2018-03-01;6(6):e13654.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Polymerization-Incompetent Uromodulin in the Pregnant Stroke-Prone Spontaneously Hypertensive Rat
Authors: S Mary, HY Small, J Siwy, W Mullen, A Giri, C Delles
Hypertension, 2017-03-27;69(5):910-918.
Species: Rat
Sample Types: Urine
Applications: Western Blot
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