Mouse VE-Cadherin Biotinylated Antibody

Catalog #: BAF1002 Datasheet / COA / SDS

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BAF1002

All VE-Cadherin Antibodies

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Mouse VE-Cadherin Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse VE-Cadherin in Western blots. In this format, less than 5% cross-reactivity with recombinant human VE‑Cadherin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VE-Cadherin
Asp46-Gln592
Accession # 2208309A

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse VE-Cadherin Fc Chimera (Catalog # 1002-VC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VE-Cadherin

The cadherin (Ca⁺⁺-dependent adherence) superfamily is a large group of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least five major subfamilies based on molecule gene structure, and/or extracellular (EC) and intracellular domains (1-4). Subfamilies include classical/type I, atypical/type II, and desmosomal-related cadherins (1-3). VE-Cadherin (vascular endothelial cadherin; also cadherin-5 and CD144) is a 125 kDa atypical/type II subfamily cadherin. Its subfamily classification is based principally on its genomic structure, as its physical structure is notably divergent from other type II subfamily members (2, 3). Mouse VE-Cadherin is synthesized as a 784 amino acid (aa) type I transmembrane (TM) preproprotein that contains a 24 aa signal peptide, a 21 aa prosequence, a 554 aa extracellular region (ECR), a 21 aa TM segment, and a 164 aa cytoplasmic domain (5, 6). The ECR contains five Ca⁺⁺-binding cadherin domains that are approximately 105 aa in length. Cadherin domains are comprised of two beta -sheets that are oriented like bread in a sandwich. Although complex, the N-terminal cadherin domain mediates trans interactions, while the internal domains contribute to cis multimerizations (7). Mouse VE-Cadherin ECR is 92%, 77%, and 73% aa identical to rat, human, and porcine VE-Cadherin ECR, respectively. VE-Cadherin is involved in the maintenance of endothelial permeability. In this regard, VE-Cadherin does not initiate new blood vessel formation; it maintains it once formed. Thus, when VE‑Cadherin is downregulated, cells part and permeability increases (8). Notably, VEGF is known to promote vascular leakage, and apparently does so by inducing a beta ‑arrestin-dependent endocytosis of VE-Cadherin (9). Part of this effect may be mediated by VE‑Cadherin itself which is reported to increase the membrane half-life of VEGF R2 (10). VE‑Cadherin acts homotypically at sites of zonula adherens. On each expressing cell, it is proposed that VE-Cadherin first forms a trimer, which then dimerizes with a trimeric counterpart in-trans. Alternatively, two cis-dimers could act in-trans to generate homotypic binding (11). In addition to cell adhesion, VE‑Cadherin also is reported to mediate TGF-beta receptor assembly. When clustered, VE-Cadherin enhances T beta RII/T beta RI assembly into an active receptor complex on endothelial cells (12). VE‑Cadherin is expressed on endothelial cells, trophoblast cells, endothelial progenitor cells, and embryonic hematopoietic cells (5, 8, 13, 14).

References

Patel, S.D. et al. (2007) Curr. Opin. Struct. Biol. 13:690.
Vestweber, D. (2008) Arterioscler. Thromb. Vasc. Biol. 28:223.
Vincent, P.A. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C987.
Cavallaro, U. et al. (2006) Exp. Cell Res. 312:659.
Breier, G. et al. (1996) Blood 87:630.
Huber, P. et al. (1996) Genomics 32:21.
Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
Crosby, C.V. et al. (2005) Blood 105:2771.
Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
Calera, M.R. et al. (2004) Exp. Cell Res. 300:248.
Hewat, E.A. et al. (2007) J. Mol. Biol. 365:744.
Rudini, N. et al. (2008) EMBO J. 27:993.
Kogata, N. et al. (2006) Circ. Res. 98:897.
Ema, M. et al. (2006) Blood 108:4018.

Long Name

Vascular Endothelium Cadherin

Entrez Gene IDs

1003 (Human); 12562 (Mouse)

Alternate Names

7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin