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Mouse VEGFR1/Flt-1 Antibody

Catalog #: MAB471
8 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB471-SP
MAB471-500
MAB471-100
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VEGFR1/Flt-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VEGFR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human VEGFR1, recombinant mouse (rm) VEGFR2, or rmVEGFR3 is observed.
Source
Monoclonal Rat IgG2A Clone # 141515
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VEGFR1
Ser27-Glu759
Accession # P35969
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse VEGFR1/Flt‑1 Fc Chimera (Catalog # 471-F1)
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of mouse thymus and kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR1/Flt-1

VEGFR1 (also called Flt-1) is one of the five receptor tyrosine kinases (RTKs) (VEGFR1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and Tie-2/Tek define a class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGFR1/Flt-1, VEGFR2/KDR/Flk-1, VEGFR3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Full length mouse VEGFR1 mRNA encodes a 1333 amino acid (aa) residue precursor with a 22 aa residue signal peptide. Mature VEGFR1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGFR1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGFR1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

References
  1. He, Y. et al. (1999) Molecular Endocrinology 13:537.
  2. Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.
Long Name
Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs
2321 (Human); 14254 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

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Citations for Mouse VEGFR1/Flt-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. VEGF receptor heterodimers and homodimers are differentially expressed in neuronal and endothelial cell types
    Authors: J Sarkar, Y Luo, Q Zhou, E Ivakhnitsk, D Lara, E Katz, MG Sun, V Guaiquil, M Rosenblatt
    PLoS ONE, 2022-07-21;17(7):e0269818.
    Species: Human, Rat
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation, Western Blot
  2. Pharmacogenetic inhibition of lumbosacral sensory neurons alleviates visceral hypersensitivity in a mouse model of chronic pelvic pain
    Authors: AX Xie, N Iguchi, TC Clarkson, AP Malykhina
    PLoS ONE, 2022-01-25;17(1):e0262769.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells
    Authors: J Falero-Per, N Sheibani, CM Sorenson
    PLoS ONE, 2020-05-04;15(5):e0232779.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells
    Authors: J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani
    PLoS ONE, 2018-10-29;13(10):e0206756.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism.
    Authors: Yang Y, Ahn Y, Chen Y, Tan X, Guo L, Gibbons D, Ungewiss C, Peng D, Liu X, Lin S, Thilaganathan N, Wistuba I, Rodriguez-Canales J, McLendon G, Creighton C, Kurie J
    J Clin Invest, 2014-04-24;124(6):2696-708.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  6. Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1.
    Authors: Mir SU, Jin L, Craven RJ
    J. Biol. Chem., 2012-03-14;287(18):14494-501.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  7. Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
    Authors: Suryawanshi A, Mulik S, Sharma S
    J. Immunol., 2011-02-16;186(6):3653-65.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  8. Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model.
    Authors: Isayeva T, Chanda D, Kallman L, Eltoum IE, Ponnazhagan S
    Cancer Res., 2007-06-15;67(12):5789-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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Mouse VEGF R1/Flt‑1 Antibody
By Anonymous on 02/24/2016
Application: IHC Sample Tested: Adult heart Species: Mouse

Frozen sections. Perfusion fixed with 2% PFA/PBS
VEGFR1 in green
VEGFR2 in yellow

Immunohistochemistry Mouse VEGF R1/Flt‑1 Antibody MAB471