Non-Human Primate Perforin Cartridge New

Catalog #: SPCKB-NP-011478 Datasheet / COA / SDS

Simple Plex Non-Human Primate Perforin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-NP-011478
SPCKA-NP-011478
SPCKC-NP-011478

Non-Human Primate Perforin Ella Assay Standard Curve

3 Images

Product Details

Reviews

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Non-Human Primate Perforin Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL)

Sensitivity

0.75 pg/mL

Assay Range

3.1 - 12,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

Specificity

Natural and recombinant Perforin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of non-human primate Perforin in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	23	25
Mean (pg/mL)	92.9	3901	90.7	4145
Standard Deviation	2.07	74.9	7.41	371
CV%	2.2	1.9	8.2	8.9

Recovery

Recovery was not necessary as Perforin shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of Perforin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.