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Normal Human IgG Control

Catalog #: 1-001-A
38 Citations 4 Reviews Datasheet / COA / SDS
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1-001-A
R&D Systems Antibodies
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Product Details
Citations (38)
FAQs
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Normal Human IgG Control Summary

Source
Polyclonal Human IgG
Purification
Protein A or G purified
Immunogen
Normal human plasma (Donor plasma tested and found negative for HBsAg, HIV-1, HIV-2 and HCV antibodies by FDA approved tests)
Formulation
10 mM Sodium Phosphate, 0.85% (w/v) Sodium Chloride, pH 7.2 (PBS) with 5% trehalose and 0.05% (w/v) Sodium Azide
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 2 mg/mL.
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Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
♦ 12 months from date of receipt at -20 to -70 °C

♦ 6 months from date of receipt at 2 to 8 °C

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Product Datasheet
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Product Specific Notices

Contains sodium azide.  Sodium azide may react with lead and copper plumbing to form explosive metal azides. Flush with large amounts of water during disposal.

Citations for Normal Human IgG Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features
    Authors: Italia, M;Salvadè, M;La Greca, F;Zianni, E;Pelucchi, S;Spinola, A;Ferrari, E;Archetti, S;Alberici, A;Benussi, A;Solje, E;Haapasalo, A;Hoffmann, D;Katisko, K;Krüger, J;Facchinetti, R;Scuderi, C;Padovani, A;DiLuca, M;Scheggia, D;Borroni, B;Gardoni, F;
    Brain, behavior, and immunity
    Species: Murine polyomavirus strain A3
    Sample Types: In Vivo
    Applications: In vivo assay
  2. Blood-brain barrier penetration of non-replicating SARS-CoV-2 and S1 Variants of Concern induce neuroinflammation which is accentuated in a mouse model of Alzheimer's disease
    Authors: MA Erickson, AF Logsdon, EM Rhea, KM Hansen, SJ Holden, WA Banks, JL Smith, C German, SA Farr, JE Morley, RR Weaver, AJ Hirsch, A Kovac, E Kontsekova, KK Baumann, MA Omer, J Raber
    Brain, Behavior, and Immunity, 2023-01-20;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Inhibition of the VEGF signaling pathway attenuates tumor?associated macrophage activity in liver cancer
    Authors: S Okikawa, Y Morine, Y Saito, S Yamada, K Tokuda, H Teraoku, K Miyazaki, S Yamashita, T Ikemoto, S Imura, M Shimada
    Oncology reports, 2022-02-16;47(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Migration Assay Control
  4. GluA3 autoantibodies induce alterations in dendritic spine and behavior in mice
    Authors: D Scheggia, J Stanic, M Italia, F La Greca, E Zianni, A Benussi, B Borroni, M Di Luca, F Gardoni
    Brain, Behavior, and Immunity, 2021-07-09;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo Control
  5. Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma
    Authors: YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang
    Nature Communications, 2021-07-02;12(1):4091.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  6. Synthesis and Biological Evaluation of Novel Isoxazole-Amide Analogues as Anticancer and Antioxidant Agents
    Authors: AM Eid, M Hawash, J Amer, A Jarrar, S Qadri, I Alnimer, A Sharaf, R Zalmoot, O Hammoudie, S Hameedi, A Mousa
    BioMed Research International, 2021-03-09;2021(0):6633297.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. High-Content Analysis-Based Sensitivity Prediction and Novel Therapeutics Screening for c-Met-Addicted Glioblastoma
    Authors: JW Oh, YJ Oh, S Han, NG Her, DH Nam
    Cancers, 2021-01-20;13(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  8. The inhibitory effect of TU-100 on hepatic stellate cell activation in the tumor microenvironment
    Authors: Y Wada, K Tokuda, Y Morine, S Okikawa, S Yamashita, T Ikemoto, S Imura, Y Saito, S Yamada, M Shimada
    Oncotarget, 2020-12-08;11(49):4593-4604.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. An allosteric peptide inhibitor of HIF-1&alpha regulates hypoxia-induced retinal neovascularization
    Authors: A Usui-Ouchi, E Aguilar, S Murinello, M Prins, ML Gantner, PE Wright, RB Berlow, M Friedlande
    Proc Natl Acad Sci U S A, 2020-10-26;0(0):.
    Species: Mouse
    Sample Types: Reference Standard
    Applications: Control
  10. Disruption of the tumour-associated EMP3 enhances erythroid proliferation and causes the MAM-negative phenotype
    Authors: N Thornton, V Karamatic, L Tilley, CA Green, CL Tay, RE Griffiths, BK Singleton, F Spring, P Walser, AG Alattar, B Jones, R Laundy, JR Storry, M Möller, L Wall, R Charlewood, CM Westhoff, C Lomas-Fran, V Yahalom, U Feick, A Seltsam, B Mayer, ML Olsson, DJ Anstee
    Nat Commun, 2020-07-16;11(1):3569.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  11. Nanoparticle interactions with immune cells dominate tumor retention and induce T cell-mediated tumor suppression in models of breast cancer
    Authors: P Korangath, JD Barnett, A Sharma, ET Henderson, J Stewart, SH Yu, SK Kandala, CT Yang, JS Caserto, M Hedayati, TD Armstrong, E Jaffee, C Gruettner, XC Zhou, W Fu, C Hu, S Sukumar, BW Simons, R Ivkov
    Sci Adv, 2020-03-25;6(13):eaay1601.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  12. Immunoglobulin G responses against falciparum malaria specific antigens are higher in children with homozygous sickle cell trait than those with normal hemoglobin
    Authors: GM Bwire, M Majigo, R Makalla, L Nkinda, A Mawazo, M Mizinduko, J Makani
    BMC Immunol., 2019-04-27;20(1):12.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Standard
  13. ITGA2 as a potential nanotherapeutic target for glioblastoma
    Authors: P Guo, A Moses-Gard, J Huang, ER Smith, MA Moses
    Sci Rep, 2019-04-17;9(1):6195.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  14. Calpain-mediated tau fragmentation is altered in Alzheimer's disease progression
    Authors: HH Chen, P Liu, P Auger, SH Lee, O Adolfsson, L Rey-Bellet, J Lafrance-V, BA Friedman, M Pihlgren, A Muhs, A Pfeifer, J Ernst, G Ayalon, KR Wildsmith, TG Beach, MP van der Br
    Sci Rep, 2018-11-13;8(1):16725.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  15. Hypoxia-induced activin A diminishes endothelial cell vasculogenic activity
    Authors: S Merfeld-Cl, H Lu, X Wu, KL March, DO Traktuev
    J. Cell. Mol. Med., 2017-08-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
  16. Comprehensive cross-clade characterization of antibody-mediated recognition, complement-mediated lysis and cell-mediated cytotoxicity of HIV-1 envelope specific antibodies towards the eradication of the HIV-1 reservoir
    Authors: S Mujib, J Liu, AKMN Rahman, JA Schwartz, P Bonner, FY Yue, MA Ostrowski
    J. Virol., 2017-07-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses
    Sci Rep, 2016-07-07;6(0):29447.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  18. Glycoprotein nonmetastatic melanoma protein B extracellular fragment shows neuroprotective effects and activates the PI3K/Akt and MEK/ERK pathways via the Na+/K+-ATPase.
    Authors: Yoko Ono, Kazuhiro Tsuruma, Masafumi Takata, Masamitsu Shimazawa, Hideaki Hara
    Scientific Reports, 2016-03-18;0(0):2045-2322.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Control
  19. Active immunization with Tocilizumab mimotopes induces specific immune responses.
    Authors: Yang L, Xing R, Li C, Liu Y, Sun L, Liu X, Wang Y
    BMC Biotechnol, 2015-06-02;15(0):46.
    Species: Bacteria - E. Coli
    Sample Types: Virus
    Applications: Isotype Control
  20. Targeting ADAM-17 with an inhibitory monoclonal antibody has antitumour effects in triple-negative breast cancer cells.
    Authors: Caiazza F, McGowan P, Mullooly M, Murray A, Synnott N, O'Donovan N, Flanagan L, Tape C, Murphy G, Crown J, Duffy M
    Br J Cancer, 2015-05-26;112(12):1895-903.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  21. Pericytes contribute to the disruption of the cerebral endothelial barrier via increasing VEGF expression: implications for stroke.
    Authors: Bai Y, Zhu X, Chao J, Zhang Y, Qian C, Li P, Liu D, Han B, Zhao L, Zhang J, Buch S, Teng G, Hu G, Yao H
    PLoS ONE, 2015-04-17;10(4):e0124362.
    Species: Mouse
    Sample Types: Whole Organism
    Applications: Control
  22. Hypoxia down-regulates expression of secretory leukocyte protease inhibitor in bronchial epithelial cells via TGF-beta1.
    Authors: Pahlman L, Jogi A, Gram M, Mori M, Egesten A
    BMC Pulm Med, 2015-03-07;15(0):19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. B7-H1-expressing antigen-presenting cells mediate polarization of protumorigenic Th22 subsets.
    Authors: Kuang D, Xiao X, Zhao Q, Chen M, Li X, Liu R, Wei Y, Ouyang F, Chen D, Wu Y, Lao X, Deng H, Zheng L
    J Clin Invest, 2014-09-17;124(10):4657-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  24. A novel murine cytomegalovirus vaccine vector protects against Mycobacterium tuberculosis.
    Authors: Beverley P, Ruzsics Z, Hey A, Hutchings C, Boos S, Bolinger B, Marchi E, O'Hara G, Klenerman P, Koszinowski U, Tchilian E
    J Immunol, 2014-07-28;193(5):2306-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  25. RepSox slows decay of CD34+ acute myeloid leukemia cells and decreases T cell immunoglobulin mucin-3 expression.
    Authors: Jajosky A, Coad J, Vos J, Martin K, Senft J, Wenger S, Gibson L
    Stem Cells Transl Med, 2014-05-22;3(7):836-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Induction and activation of human Th17 by targeting antigens to dendritic cells via dectin-1.
    Authors: Duluc D, Joo H, Ni L, Yin W, Upchurch K, Li D, Xue Y, Klucar P, Zurawski S, Zurawski G, Oh S
    J Immunol, 2014-05-16;192(12):5776-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  27. Pathogenic strains of Acanthamoeba are recognized by TLR4 and initiated inflammatory responses in the cornea.
    Authors: Alizadeh H, Tripathi T, Abdi M, Smith A
    PLoS ONE, 2014-03-14;9(3):e92375.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  28. The aryl hydrocarbon receptor is functionally upregulated early in the course ofvhuman T-cell activation.
    Authors: Prigent L, Robineau M, Jouneau S, Morzadec C, Louarn L, Vernhet L, Fardel O, Sparfel L
    Eur J Immunol, 2014-02-18;44(5):1330-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  29. Generation of neuronal progenitor cells in response to tumors in the human brain.
    Authors: Macas J, Ku M, Nern C, Xu Y, Buhler H, Remke M, Synowitz M, Franz K, Seifert V, Plate K, Kettenmann H, Glass R, Momma S
    Stem Cells, 2014-01-01;32(1):244-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  30. Angiogenesis stimulated by human kallikrein-related peptidase 12 acting via a platelet-derived growth factor B-dependent paracrine pathway.
    Authors: Kryza T, Achard C, Parent C, Marchand-Adam S, Guillon-Munos A, Iochmann S, Korkmaz B, Respaud R, Courty Y, Heuze-Vourc'h N
    FASEB J, 2013-11-13;28(2):740-51.
    Species: Human
    Sample Types: Protein, Whole Cells
    Applications: IHC, Western Blot
  31. Anti-Tumour Effects of a Specific Anti-ADAM17 Antibody in an Ovarian Cancer Model In Vivo.
    Authors: Richards FM, Tape CJ, Jodrell DI, Murphy G
    PLoS ONE, 2012-07-11;7(7):e40597.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  32. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012-06-15;287(32):26478-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  33. Sjogren's syndrome pathological neovascularization is regulated by VEGF-A-stimulated TACE-dependent crosstalk between VEGFR2 and NF-kappaB.
    Authors: Sisto M, Lisi S, Lofrumento DD, D'Amore M, Frassanito MA, Ribatti D
    Genes Immun., 2012-04-19;13(5):411-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus.
    Authors: Noyce RS, Bondre DG, Ha MN
    PLoS Pathog., 2011-08-25;7(8):e1002240.
    Species: Hamster, Human
    Sample Types: Whole Cells
    Applications: Fc Blocking
  35. Inhibition of Cell Proliferation by an Anti-EGFR Aptamer.
    Authors: Li N, Nguyen HH, Byrom M, Ellington AD
    PLoS ONE, 2011-06-08;6(6):e20299.
    Species: Human
    Sample Types: RNA
    Applications: Control
  36. HER2 phosphorylation is maintained by a PKB negative feedback loop in response to anti-HER2 herceptin in breast cancer.
    Authors: Gijsen M, King P, Perera T, Parker PJ, Harris AL, Larijani B, Kong A
    PLoS Biol., 2010-12-21;8(12):e1000563.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  37. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
    Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
    J. Immunol., 2010-10-22;185(11):7047-56.
    Species: Human
    Sample Types: Whole Cells
  38. TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche.
    Authors: Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H
    Cancer Res., 2008-12-01;68(23):9754-62.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control

FAQs

  1. What subtype of IgG does Normal Human IgG Control (Catalog # 1-001-A) contain?

    • Normal Human IgG Control (Catalog # 1-001-A) is produced by purification of IgG from Normal human plasma. It may contain more than one subtype of IgG.

View all Isotype Control FAQs

Reviews for Normal Human IgG Control

Average Rating: 5 (Based on 4 Reviews)

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Normal Human IgG Control
By Anonymous on 02/16/2020
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

IgG was used as negative control.

Western Blot Normal Human IgG Control 1-001-A
Normal Human IgG Control
By Alberto Contreras on 02/04/2020
Application: Func Sample Tested: Bladder cancer tissue Species: Human

UM-UC3 bladder cancer cells were transiently transfected with a Notch-reporter Firefly luciferase plasmid, in addition to an internal Renilla control. IgG was used as a negative stimulation control, whereas Jagged 1 was used to induce a Notch-specific response. Increasing concentrations of the Gamma-secretase inhibitor DAPT resulted in a dose-dependent decrease in Notch signal.

Functional Assay Normal Human IgG Control 1-001-A
Normal Human IgG Control
By Anonymous on 09/22/2019
Application: WB Sample Tested: Recombinant protein Species: Human
Western Blot Normal Human IgG Control 1-001-A
Normal Human IgG Control
By Anonymous on 10/27/2018
Application: Flow Sample Tested: MBA-MB-123 human breast cancer cell line Species: Human

Used at 10 ug/mL works perfectly as a human Isotype control for flow cytometry. There is no background binding in live cells that do not express Fc receptor. The attached image shows MFI with and without the isotype control indicating identical signal.

Flow Cytometry Normal Human IgG Control 1-001-A