NucA nuclease Antibody

Catalog #: MAB100633 Datasheet / COA / SDS

Detects Benzonase®. Benzonase® is a registered trademark of Merck KGaA Corporation. Bio-Techne Corporation and its affiliates, and their respective products and services, are not sponsored, endorsed, or approved by Merck KGaA Corporation

Catalog #	Availability	Size / Price	Qty
MAB100633-100
MAB100633-SP

Detection of NucA nuclease by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

NucA nuclease Antibody Summary

Specificity

Detects Benzonase® Nuclease/NucA in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 1028905

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived s. marcescens Benzonase Nuclease/NucA
Met1-Asn266
Accession # P13717

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Recombinant NucA nuclease Protein

Simple Western

10 µg/mL

Recombinant NucA nuclease Protein

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of NucA nuclease by Western Blot. Western blot shows recombinant NucA nuclease protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100633) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NucA nuclease at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of NucA nuclease by Simple Western^TM. Simple Western lane view shows recombinant NucA nuclease protein, loaded at 0.2 mg/mL. A specific band was detected for NucA nuclease at approximately 35 kDa (as indicated) using 10 µg/mL of Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100633). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NucA nuclease

Serratia marcescens endonuclease, also known as Benzonase, is a non-specific nuclease that degrades both single- and double-stranded nucleic acids, including DNA and RNA, but exhibits no proteolytic activity (1); therefore, it is ideal for the removal of nucleic acid contaminants from protein samples and applications where complete digestion of nucleic acids is desirable. NucA (Benzonase) is also commonly used in bioprocessing applications to reduce viscosity of samples caused by genomic DNA. The optimum pH for enzyme activity is found to be 8.0-9.2. It hydrolyzes internal phosphodiester bonds between nucleotides in nucleic acids to produce 5'-monophosphate oligonucleotides of 3-8 bases in length (2). The active enzyme is a homodimer with two disulfide bonds in each monomer that are crucial to the enzyme activity and stability (3). The absolute activity of the recombinant enzyme is measured by a phosphatase coupled assay (4), where the 5'-phosphate of oligosaccharides generated by the enzyme is further released by non-specific alkaline phosphatase and quantitated by Malachite reagents (5).

References