PA Janelia Fluor® 549, SE
Chemical Name: 2,5-Dioxo-1-pyrrolidinyl 3',6'-di-1-azetidinyl-2-diazo-2,3-dihydro-3-oxospiro[1H-indene-1,9'-[9H]xanthene]-6-carboxylate
Purity: ≥90%
Biological Activity
PA Janelia Fluor® 549, SE is a photoactivatable fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Non-fluorescent until activated at 365 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. Can be multiplexed with PA Janelia Fluor® 646, SE (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Cell permeable. Excitation maximum = 551 - 553 nm; emission maximum = 570 - 573 nm.Please see the protocol for further information on protein/antibody labeling and conjugation.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
Background References
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Bright photoactivatable fluorophores for single-molecule imaging.
Grimm et al.
Nat.Methods, 2016;13:985 -
Real-time imaging of Huntingtin aggregates diverting target search and gene transcription.
Li et al.
eLife, 2016;5:e17056 -
Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome.
Hong et al.
Proc.Natl.Acad.Sci.USA., 2009;106:14276
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Citation for PA Janelia Fluor® 549, SE
The citations listed below are publications that use Tocris products. Selected citations for PA Janelia Fluor® 549, SE include:
1 Citation: Showing 1 - 1
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Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
Authors: Genshaft Et al.
Nat Commun 2021;12:4995
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