Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

Catalog #: IC3570F
2 Citations Datasheet / COA / SDS

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IC3570F

Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry.

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Citations (2)

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Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human EGF R phosphorylated at Y1068 in flow cytometry.

Source

Monoclonal Mouse IgG_2A Clone # 338324

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing human EGF R Y1068 site

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of EGF R/ErbB1 antibody in A431 Human Cell Line antibody by Flow Cytometry.

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Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line treated with recombinant human EGF (Catalog # 236-EG) was stained with Mouse Anti-Human Phospho-EGF R/ErbB1 (Y1068) Fluorescein-conjugated Monoclonal Antibody (Catalog # IC3570F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: EGFR

Epidermal growth factor receptor (EGF R, also known as ErbB1 and HER1) is the founding member of the ErbB family of receptor tyrosine kinases. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation at Tyr 1068 allows binding of the SH2 domain of the cytosolic adaptor Grb2. This binding results in Ras activation.

Long Name

Epidermal Growth Factor Receptor

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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Product Datasheet

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Citations for Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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In situ neutrophil efferocytosis shapes T cell immunity to influenza infection
Authors: K Lim, TH Kim, A Trzeciak, AM Amitrano, EC Reilly, H Prizant, DJ Fowell, DJ Topham, M Kim
Nat. Immunol., 2020-08-03;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Loss of N-Acetylgalactosaminyltransferase-4 Orchestrates Oncogenic MicroRNA-9 in Hepatocellular Carcinoma
Authors: Y Liu, H Liu, L Yang, Q Wu, W Liu, Q Fu, W Zhang, H Zhang, J Xu, J Gu
J. Biol. Chem, 2017-01-06;292(8):3186-3200.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry