Phospho-Synaptotagmin‑1 (T202) Antibody

Catalog #: PPS086 Datasheet / COA / SDS

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PPS086

Detection of Phospho-Synaptotagmin-1 (T202) by Western Blot.

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Phospho-Synaptotagmin‑1 (T202) Antibody Summary

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Zebrafish

Specificity

Rat Synaptotagmin phosphorylated at T202

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-T202 of Synaptotagmin-1

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Phospho-Synaptotagmin-1 (T202) by Western Blot. Western Blot of rat cortex lysate showing specific immunolabeling of the ~60 - 62 kDa Synaptotagmin phosphorylated at T202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase; lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to anti-Synaptotagmin (T202). The immunolabeling is completely eliminated by treatment with lambda PPase.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Synaptotagmin-1

Synaptotagmins are integral membrane proteins of synaptic vesicles. Synaptotagmin-1 is a glycoprotein containing two C2 domains related to protein kinase C and sites for calcium-dependent binding of acidic phospholipids. Synaptotagmin-1 participates in the process of vesicular trafficking and exocytosis by inducing local calcium-dependent buckling of the plasma membrane. Synaptotagmin-2 is a single-pass, type Ia/III (no signal sequence) transmembrane (TM) glycoprotein. Synaptotagmin-2 is an integral component of neurotransmitter-containing synaptic vesicles that detects action potential-induced increases in presynaptic cytosolic calcium. Increased ionic calcium binds to synaptotagmin II at two sites (C2a and C2b) on its cytoplasmic tail. The first site also binds phospholipid, while the second site binds syntaxin. This promotes vesicle membrane fusion with the presynaptic plasma membrane, resulting in neurotransmitter release.

Synaptotagmins undergo three types of posttranslational modification that may affect function. N-linked glycosylation and/or O-linked glycosylation are likely necessary for recycling (internalization) of vesicle membrane after neurotransmitter release. Fatty acylation/palmitoylation of synaptotagmin may be necessary for proper cycling. Finally, synaptoagmin phosphorylation within the C2a site regulates calcium-binding, while phosphorylation in the C2b site may regulate calcium and syntaxin interaction.

Entrez Gene IDs

6857 (Human); 20979 (Mouse); 25716 (Rat)

Alternate Names

DKFZp781D2042; P65; SVP65; synaptotagmin Isynaptotagmin 1; Synaptotagmin1; Synaptotagmin-1; SYT; SYT1; sytI