Porcine IL‑12/IL‑23 p40 Biotinylated Antibody

Catalog #: BAM9122
1 Citation Datasheet / COA / SDS

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BAM9122

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine IL‑12/IL‑23 p40 Biotinylated Antibody Summary

Species Reactivity

Porcine

Specificity

Detects the p40 subunit of porcine IL-12 and IL-23. In ELISAs, this antibody shows 25-35% cross-reactivity with recombinant human (rh) IL‑12 and rhIL‑23 and no cross-reactivity with rmIL-12 or rmIL-23.

Source

Monoclonal Mouse IgG_2B Clone # 116211

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p70 heterodimer

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Porcine IL-12 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Porcine IL‑12/IL‑23 p40 Antibody (Catalog # MAB9121)

Standard: Recombinant Porcine IL-12 Protein (Catalog # 912-PL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-23 p40

IL-12 and IL-23 are disulfide-linked heterodimers consisting of a p40 subunit that is common to both cytokines and a unique subunit, p19 in IL-23 or p35 in IL-12.

Long Name

Interleukin 12/Interleukin 23 p40

Entrez Gene IDs

3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)

Alternate Names

CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Porcine IL‑12/IL‑23 p40 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets
Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts
Infect. Immun., 2017-03-23;85(4):.
Species: Porcine
Sample Types: Serum
Applications: ELISA Development (Detection)