Porcine IL-4 Antibody

Catalog #: AF654
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF654
AF654-SP

Cell Proliferation Induced by IL‑4 and Neutralization by Porcine IL‑4 Antibody.

2 Images

All IL-4 Antibodies

Product Details

Citations (4)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine IL-4 Antibody Summary

Species Reactivity

Porcine

Specificity

Detects porcine IL-4 in ELISAs and Western blots. In direct ELISAs, this antibody shows less than 10% cross-reactivity with recombinant mouse IL‑4 and recombinant rat IL‑4.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant porcine IL-4
His25-Cys133
Accession # Q04745

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Porcine IL‑4 (Catalog # 654-P4)

Immunocytochemistry

5-15 µg/mL

See below

Porcine IL-4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Porcine IL‑4 Biotinylated Antibody (Catalog # BAF654)

Standard: Recombinant Porcine IL-4 Protein (Catalog # 654-P4)

Neutralization

Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 2 ng/mL Recombinant Porcine IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

View Larger

Cell Proliferation Induced by IL‑4 and Neutralization by Porcine IL‑4 Antibody. Recombinant Porcine IL-4 (Catalog # 654-P4) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-4 (2 ng/mL) is neutralized (green line) by increasing concen-trations of Porcine IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654). The ND₅₀ is typically 1-5 µg/mL.

Immunocytochemistry

View Larger

IL‑4 in Porcine PBMCs. IL-4 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Porcine IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Porcine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature porcine IL-4 shares 78%, 59%, 41%, and 41% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human IL-4 is active on porcine vascular endothelial cells (5). IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8, 11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

References

Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
Bailey, M. et al. (1993) Biochim. Biophys. Acta 1171:328.
Redfield, C. et al. (1991) Biochemistry 30:11029.
Stocker, C.J. et al. (2000) J. Immunol. 164:3309.
Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
Corthay, A. (2006) Scand. J. Immunol. 64:93.
Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)

Alternate Names

B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Porcine IL-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines
Authors: SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella
MethodsX, 2019-05-17;6(0):1218-1227.
Species: Porcine
Sample Types: Tissue Homogenates
Applications: ELISA Capture
Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets
Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts
Infect. Immun., 2017-03-23;85(4):.
Species: Porcine
Sample Types: Serum
Applications: ELISA Development (Capture)
Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
Authors: Nadworny PL, Wang J, Tredget EE
J Inflamm (Lond), 2010-02-19;7(0):13.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-P
Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
Authors: Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
J. Virol., 2006-01-01;80(1):372-82.
Species: Porcine
Sample Types: Whole Cells
Applications: ELISpot Development