Propidium iodide

Catalog # Availability Size / Price Qty
5135/10
Propidium iodide | CAS No. 25535-16-4 | Fluorescent DNA Stains
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Description: Red-fluorescent DNA stain, membrane impermeant to live cells. Used for: apoptosis detection, nuclear counterstaining, viability staining. Application: flow cytometry, confocal microscopy, fluorescence microscopy
Alternative Names: PI

Chemical Name: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide

Purity: ≥95%

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Biological Activity

Key information: Propidium iodide (PI) is a widely used red-fluorescent DNA stain. It is membrane impermeant to live cells and so PI staining differentiates live and dead cells. PI can be used to stain nucleic acids in dead cells across different cell types, including mammalian cells, bacteria, and yeast.

Application: flow cytometry, confocal microscopy, fluorescence microscopy.

Used for: apoptosis detection by PI staining, nuclear counterstaining in multicolor fluorescent assays, cell death measuring in a mixed live-cell population by PI staining and flow cytometry.

Properties and Photophysical Data: Propidium iodide fluorescence increases by approximately 20- to 30-fold upon binding to DNA or RNA (through intercalation). When unbound, excitation and emission maxima (λ) are 493 nm and 636 nm, respectively, when bound to DNA, excitation and emission maxima (λ) are 535 nm and 617 nm, respectively.

Optical Data for Propidium iodide

Propidium iodide Dye Spectra
Emission Color 橘黄
λabs 535 nm
λem 617 nm
Application Flow Cytometry, Fluorescence Microscopy, Confocal Microscopy

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Technical Data

M.Wt:
668.39
Formula:
C27H34I2N4
Solubility:
Soluble to 5 mM in water with sonication
Purity:
≥95%
Storage:
Store at -20°C
CAS No:
25535-16-4

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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