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Proteome Profiler Mouse XL Cytokine Array

Catalog #: ARY028
136 Citations 35 Reviews Datasheet / COA / SDS
Contains 4 membranes-each spotted in duplicate with 111 different cytokine antibodies
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ARY028
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Detection of selected mouse cytokines in Balb/3T3 Cell Line Lysate
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Citations (136)
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Proteome Profiler Mouse XL Cytokine Array Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and urine.

Troubleshooting Guide

Key Benefits

  • Detects 111 mouse cytokines simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are required.  Refer to protocol.
 
 

Principle of the Assay

The Proteome Profiler Mouse XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Mouse XL Cytokine Array nitrocellulose membranes spotted with 111 different antibodies to mouse cytokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Mouse XL Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

 
Simultaneously detect the levels of these cytokines, chemokines, and growth factors in a single sample.
Adiponectin/Acrp30 DPPIV/CD26 IL-27
Amphiregulin EGF IL-28
Angiopoietin-1 Endoglin/CD105 IL-33
Angiopoietin-2 Endostatin LDL R
Angiopoietin-like 3 Fetuin A/AHSG Leptin
BAFF/BLyS/TNFSF13B FGF acidic LIF
C1q R1/CD93 FGF-21 Lipocalin-2/NGAL,
CCL2/JE/MCP-1 Flt-3 Ligand LIX
CCL3/CCL4 MIP-1 alpha/beta Gas6 M-CSF
CCL5/RANTES G-CSF MMP-2
CCL6/C10 GDF-15 MMP-3
CCL11/Eotaxin GM-CSF MMP-9
CCL12/MCP-5 HGF Myeloperoxidase
CCL17/TARC ICAM-1/CD54 Osteopontin (OPN)
CCL19/MIP-3 beta IFN-gamma Osteoprotegerin/TNFRSF11B
CCL20/MIP-3 alpha IGFBP-1 PD-ECGF/Thymidine phosphorylase
CCL21/6Ckine IGFBP-2 PDGF-BB
CCL22/MDC IGFBP-3 Pentraxin 2/SAP
CD14 IGFBP-5 Pentraxin 3/ TSG-14
CD40/TNFRSF5 IGFBP-6 Periostin/OSF-2
CD160 IL-1 alpha/IL1F1 Pref-1/DLK-1/FA1
Chemerin IL-1 beta/IL-1F2 Proliferin
Chitinase 3-like 1 IL-1ra/IL-1F3 Proprotein Convertase 9/PCSK9
Coagulation Factor III/Tissue Factor IL-2 RAGE
Complement Component C5/C5a IL-3 RBP4
Complement Factor D IL-4 Reg3G
C-Reactive Protein/CRP IL-5 Resistin
CX3CL1/Fractalkine IL-6 E-Selectin/CD62E
CXCL1/KC IL-7 P-Selectin/CD62P
CXCL2/MIP-2 IL-10 Serpin E1/PAI-1
CXCL9/MIG IL-11 Serpin F1/PEDF
CXCL10/IP-10 IL-12p40 Thrombopoietin
CXCL11/I-TAC IL-13 TIM-1/KIM-1/HAVCR
CXCL13/BLC/BCA-1 IL-15 TNF-alpha
CXCL16 IL-17A VCAM-1/CD106
Cystatin C  IL-22 VEGF
Dkk-1 IL-23 WISP-1/CCN4

Assays for Analytes represented in the Mouse XL Cytokine Array Kit

Analyte Qkit Duoset
Adiponectin/Acrp30 MRP300   DY1119  
Amphiregulin     DY989  
Angiopoietin-1        
Angiopoietin-2        
Angiopoietin-like 3 MANL30   DY136  
BAFF/BLyS/TNFSF13B MBLYS0   DY2106-05  
C1q R1/CD93 MCD930      
CCL2/JE/MCP-1 MJE00   DY479  
CCL3/CCL4 MIP-1 alpha/beta        
CCL5/RANTES MMR00   DY478  
CCL6/C10     DY487  
CCL11/Eotaxin MME00   DY420  
CCL12/MCP-5 MCC120   DY428  
CCL17/TARC MCC170   DY529  
CCL19/MIP-3 beta     DY440  
CCL20/MIP-3 alpha MCC200   DY760  
CCL21/6Ckine     DY457  
CCL22/MDC MCC220   DY439  
CD14 MC140   DY982  
CD40/TNFRSF5 MCCD40   DY097  
CD160 MCHM00   DY2325  
Chemerin        
Chitinase 3-like 1 MC3L10   DY2649  
Coagulation Factor III/Tissue Factor        
Complement Component C5/C5a        
Complement Factor D        
C-Reactive Protein/CRP MCRP00   DY1829  
CX3CL1/Fractalkine MCX310   DY472  
CXCL1/KC MKC00B   DY453  
CXCL2/MIP-2 MM200   DY452  
CXCL9/MIG MCX900   DY492  
CXCL10/IP-10 MCX100   DY466  
CXCL11/I-TAC     DY572  
CXCL13/BLC/BCA-1 MCX130      
CXCL16     DY503  
Cystatin C  MSCTC0   DY1238  
Dkk-1 MKK100   DY1765  
DPPIV/CD26     DY954  
EGF MEG00   DY2028  
Endoglin/CD105 MNDG00   DY1320  
Endostatin        
Fetuin A/AHSG MFTA00   DY1563  
FGF acidic     DY4686-05  
FGF-21 MF2100   DY3057  
Flt-3 Ligand MFK00   DY427  
Gas6 MGAS60   DY986  
G-CSF MCS00   DY414  
GDF-15 MGD150   DY6385  
GM-CSF MGM00   DY415  
HGF MHG00   DY2207  
ICAM-1/CD54 MIC100   DY796  
IFN-gamma MIF00   DY485  
IGFBP-1     DY1588-05  
IGFBP-2     DY797  
IGFBP-3 MGB300   DY775  
IGFBP-5     DY578  
IGFBP-6     DY776  
IL-1 alpha/IL1F1 MLA00   DY400  
IL-1 beta/IL-1F2 MLB00C   DY401  
IL-1ra/IL-1F3 MRA00   DY480  
IL-2 M2000   DY402  
IL-3 M3000   DY403  
IL-4 M4000B   DY404  
IL-5 M5000   DY405  
IL-6 M6000B   DY406  
IL-7 M7000   DY407  
IL-10 M1000B   DY417  
IL-11     DY418  
IL-12p40 MP400
non-allele specific		 M1240
allele specific		 DY2398
non-allele specific		 DY499
allele specific
IL-13 M1300CB   DY413  
IL-15     DY447  
IL-17A M1700   DY421  
IL-22 M2200   DY582  
IL-23 M2300   DY1887  
IL-27     DY1789B  
IL-28        
IL-33 M3300   DY3626  
LDL R MLDLR0   DY2255  
Leptin MOB00   DY498  
LIF MLF00      
Lipocalin-2/NGAL,     DY1857  
LIX MX000   DY443  
M-CSF MMC00   DY416  
MMP-2 MMP200      
MMP-3 MMP300   DY548  
MMP-9 MMPT90 MMP900B DY6718 DY909
Myeloperoxidase     DY3667  
Osteopontin (OPN) MOST00   DY441  
Osteoprotegerin/TNFRSF11B MOP00   DY459  
PD-ECGF/Thymidine phosphorylase        
PDGF-BB MBB00      
Pentraxin 2/SAP MPTX20      
Pentraxin 3/ TSG-14 MPTX30   DY2166  
Periostin/OSF-2 MOSF20   DY2955  
Pref-1/DLK-1/FA1        
Proliferin        
Proprotein Convertase 9/PCSK9 MPC900   DY3985  
RAGE MRG00   DY1179  
RBP4 MRBP40   DY3476  
Reg3G        
Resistin MRSN00   DY1069  
E-Selectin/CD62E MES00   DY575  
P-Selectin/CD62P MPS00   DY737  
Serpin E1/PAI-1     DY3828-05  
Serpin F1/PEDF        
Thrombopoietin MTP00   DY488  
TIM-1/KIM-1/HAVCR MKM100   DY1817  
TNF-alpha MTA00B   DY410  
VCAM-1/CD106 MVC00   DY643  
VEGF MMV00   DY493  
WISP-1/CCN4 MWSP10      

Specifications

Source
N/A
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Mouse

Product Datasheets

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Scientific Data

N/A Detection of selected mouse cytokines in Balb/3T3 Cell Line Lysate View Larger

Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Lysate. Lysates from Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) for 24 hours (200 µg lysate, 10 minute exposure).

N/A Detection of Selected Mouse Cytokines in CMT-93 Cell Line Lysate. View Larger

Detection of Selected Mouse Cytokines in CMT-93 Cell Line Lysate. Lysates from CMT-93 mouse rectal carcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (200 µg lysate, 10 minute exposure).

N/A Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Supernate. View Larger

Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Supernate. Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) for 24 hours (500 µL of cell culture supernate, 5 minute exposure).

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, relative expression levels of mouse cytokines and chemokines in samples can be determined using the following procedure:

  • Prepare membrane and incubate with prepared sample
  • Incubate the membrane with Detection Antibody Cocktail
  • Incubate the membrane array with Streptavidin-HRP
  • Develop the membrane array with Chemi Reagents 1 and 2
  • Expose the membrane array to autoradiography film

View Graphic Procedure
 

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Mouse XL Cytokine Array nitrocellulose membranes spotted with 111 different antibodies to mouse cytokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Mouse XL Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

 

Other Supplies Required

Reagents

Materials

  • Pipettes and pipette tips
  • Gloves
  • Plastic container with the capacity to hold 50 mL (for washing the arrays)
  • Plastic transparent sheet protector (trimmed to 10 cm x 12 cm and open on three sides)
  • Plastic wrap
  • Absorbent lab wipes (KimWipes® or equivalent)
  • Paper towels
  • X-ray film (Kodak BioMax™ Light-1) or equivalent
  • Flat-tipped tweezers

Equipment

  • Rocking platform shaker
  • Microcentrifuge
  • Autoradiography cassette
  • Film developer
  • Flatbed scanner with transparency adapter capable of transmission mode
  • Computer capable of running image analysis software and Microsoft Excel

Other Supplies Required for Cell Lysate Samples

  • Phosphate-Buffered Saline (PBS)
  • Lysis buffer (1% Igepal CA-630, 20 mM Tris-HCl (pH 8.0), 137 mM NaCl, 10% glycerol, 2 mM EDTA, 10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)

Other Supplies Required for Tissue Lysate Samples

  • PBS with protease inhibitors (10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)
  • Triton™ X-100

 

Procedure Overview

R&D Systems Protocol for Multiple Analyte Detection Using the Proteome Profiler™ Mouse XL Cytokine Array Kit, Panel A (Catalog # ARY028)

Add 2 mL of Array Buffer 6 to each well of the supplied 4-Well Multi-dish.

Add 2 mL of Array Buffer 6 to each well of the supplied 4-Well Multi-dish.

Place each array membrane in a separate well of the 4-Well Multi-dish.

Incubate for one hour on a rocking platform shaker.

Place each array membrane in a separate well of the 4-Well Multi-dish

Add 0.5 mL Array Buffer 4 to each sample.

Adjust volume of each sample to final volume of 1.5 mL with Array Buffer 6.

Add 0.5 mL Array Buffer 4 to each sample.

Replace the Array Buffer 6 in each well of the 4-Well Multi-dish with prepared samples.

Incubate overnight at 2 °C to 8 °C on a rocking platform.

Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.

Add 0.5 mL Array Buffer 4 to each sample.
Add 30 µL of Detection Antibody Cocktail to 1.5 mL of Array Buffer 4/6 for each array.
Add 0.5 mL Array Buffer 4 to each sample.

Pipette 1.5 mL diluted Detection Antibody Cocktail into each well of the 4-Well Multi-dish.

Add 0.5 mL Array Buffer 4 to each sample.

Return each array membrane to the 4-Well Multi-dish containing diluted Detection Antibody Cocktail.

Incubate for one hour on a rocking platform shaker.

Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.

Add 0.5 mL Array Buffer 4 to each sample.

Add 2 mL of diluted Streptavidin-HRP to each well of the 4-Well Multi-dish.

Place the array membrane in the diluted Streptavidin-HRP solution.

Incubate for 30 minutes on a rocking platform shaker.

Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

Add 0.5 mL Array Buffer 4 to each sample.

Place the array membrane on a plastic sheet protector.

Pipette 1 mL of the prepared Chemi Reagent Mix evenly onto the membrane.

Cover the membrane with the top sheet of the plastic protector.

Incubate for 1 minute.

Blot off excess Chemi Reagent Mix.

Wrap the membrane and sheet protector in plastic wrap.

Place the wrapped array membrane in an autoradiography film cassette and expose to X-ray film.

Add 0.5 mL Array Buffer 4 to each sample.

Citations for Proteome Profiler Mouse XL Cytokine Array

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

136 Citations: Showing 1 - 10
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  1. Induced electric fields inhibit breast cancer growth and metastasis by modulating the immune tumor microenvironment
    Authors: Charan, M;Jones, TH;Ahirwar, DK;Acharya, N;Subramaniam, VV;Ganju, RK;Song, JW;
    bioRxiv : the preprint server for biology  2024-04-17
  2. Parotid glands have a dysregulated immune response following radiation therapy
    Authors: Gunning, JA;Gilman, KE;Zúñiga, TM;Simpson, RJ;Limesand, KH;
    PloS one  2024-03-12
  3. Mycoplasma DnaK expression increases cancer development in vivo upon DNA damage
    Authors: Benedetti, F;Silvestri, G;Denaro, F;Finesso, G;Contreras-Galindo, R;Munawwar, A;Williams, S;Davis, H;Bryant, J;Wang, Y;Radaelli, E;Rathinam, CV;Gallo, RC;Zella, D;
    Proceedings of the National Academy of Sciences of the United States of America  2024-03-05
  4. Deciphering Early-Stage Molecular Mechanisms of Negative Pressure Wound Therapy in a Murine Model
    Authors: Shyu, YC;Huang, TS;Chiu, HS;Sumazin, P;Lin, XY;Liao, PC;Liou, CC;Hsu, FC;Lin, JS;Hsu, CC;Hsu, PH;Sun, CC;Chen, CT;
    International journal of molecular sciences  2024-02-17
  5. Eosinophils preserve bone homeostasis by inhibiting excessive osteoclast formation and activity via eosinophil peroxidase
    Authors: Andreev, D;Kachler, K;Liu, M;Chen, Z;Krishnacoumar, B;Ringer, M;Frey, S;Krönke, G;Voehringer, D;Schett, G;Bozec, A;
    Nature communications  2024-02-05
  6. Enhancing anti-tumor potential: low-intensity vibration suppresses osteosarcoma progression and augments MSCs' tumor-suppressive abilities
    Authors: Xiong, X;Huo, Q;Li, K;Cui, C;Chang, C;Park, C;Ku, B;Hong, CS;Lim, H;Pandya, PH;Saadatzadeh, MR;Bijangi-Vishehsaraei, K;Lin, CC;Kacena, MA;Pollok, KE;Chen, A;Liu, J;Thompson, WR;Li, XL;Li, BY;Yokota, H;
    Theranostics  2024-01-27
  7. Activation and Functions of Col6a1+ Fibroblasts in Colitis-Associated Cancer
    Authors: Chalkidi, N;Melissari, MT;Henriques, A;Stavropoulou, A;Kollias, G;Koliaraki, V;
    International journal of molecular sciences  2023-12-21
  8. Krüppel-like Factor 5 Plays an Important Role in the Pathogenesis of Chronic Pancreatitis
    Authors: Alavi, M;Mejia-Bautista, A;Tang, M;Bandovic, J;Rosenberg, AZ;Bialkowska, AB;
    Cancers  2023-11-15
  9. CXCL9 recombinant adeno-associated virus (AAV) virotherapy sensitizes glioblastoma (GBM) to anti-PD-1 immune checkpoint blockade
    Authors: von Roemeling, C;Yegorov, O;Yang, C;Klippel, K;Russell, R;Trivedi, V;Bhatia, A;Doonan, B;Carpenter, S;Ryu, D;Grippen, A;Futch, H;Ran, Y;Hoang-Minh, L;Weidert, F;Golde, T;Mitchell, D;
    Research square  2023-11-14
  10. The microRNA-mediated gene regulatory network in the hippocampus and hypothalamus of the aging mouse
    Authors: Munkhzul, C;Yi, SS;Kim, J;Lee, S;Kim, H;Moon, JS;Lee, M;
    PloS one  2023-11-09
  11. Senescent Schwann cells induced by aging and chronic denervation impair axonal regeneration following peripheral nerve injury
    Authors: Fuentes-Flores, A;Geronimo-Olvera, C;Girardi, K;Necuñir-Ibarra, D;Patel, SK;Bons, J;Wright, MC;Geschwind, D;Hoke, A;Gomez-Sanchez, JA;Schilling, B;Rebolledo, DL;Campisi, J;Court, FA;
    EMBO molecular medicine  2023-10-20
  12. N-acetyl cysteine mitigates lung damage and inflammation after chlorine exposure in vivo and ex vivo
    Authors: Gustafson, Å;Elfsmark, L;Karlsson, T;Jonasson, S;
    Toxicology and applied pharmacology  2023-10-10
  13. Notch3 deletion regulates HIV-1 gene expression and systemic inflammation to ameliorate chronic kidney disease
    Authors: Thornton, M;Sommer, N;McGonigle, M;Ram, AK;Yerrathota, S;Ehirim, H;Chaturvedi, A;Phan, JD;Chakravarthi, PV;Gunewardena, S;Talreja, J;Singhal, P;Fields, TA;Ray, PE;Dhillon, NK;Sharma, M;
    bioRxiv : the preprint server for biology  2023-09-13
  14. Heparanase 2 (Hpa2)- a new player essential for pancreatic acinar cell differentiation
    Authors: Kayal, Y;Barash, U;Naroditsky, I;Ilan, N;Vlodavsky, I;
    Cell death & disease  2023-07-25
  15. Mechanisms of Innate Immune Injury in Arrhythmogenic Cardiomyopathy
    Authors: Chelko, SP;Penna, V;Engel, M;Landim-Vieira, M;Cannon, EN;Lavine, K;Saffitz, JE;
    bioRxiv : the preprint server for biology  2023-07-13
  16. Loss of epidermal glucocorticoid receptor protects against whole body metabolic dysfunction upon chronic corticosterone treatment
    Authors: Gallego, A;Pérez, P;
    Molecular metabolism  2023-06-24
  17. Aging modulates homeostatic leukocyte trafficking to the peritoneal cavity in a sex-specific manner
    Authors: Hopkin, SJ;Pezhman, L;Begum, J;Kavanagh, D;McGettrick, HM;Iqbal, AJ;Chimen, M;
    Journal of leukocyte biology  2023-06-13
  18. CD4+ T cells aggravate hemorrhagic brain injury
    Authors: Shi, SX;Xiu, Y;Li, Y;Yuan, M;Shi, K;Liu, Q;Wang, X;Jin, WN;
    Science advances  2023-06-09
  19. The soluble guanylyl cyclase pathway is inhibited to evade androgen deprivation-induced senescence and enable progression to castration resistance
    Authors: Zhang, L;Troccoli, CI;Mateo-Victoriano, B;Lincheta, LM;Jackson, E;Shu, P;Plastini, T;Tao, W;Kwon, D;Chen, X;Sharma, J;Jorda, M;Gulley, JL;Bilusic, M;Lockhart, AC;Beuve, A;Rai, P;
    bioRxiv : the preprint server for biology  2023-05-03
  20. Metabololipidomic and proteomic profiling reveals aberrant macrophage activation and interrelated immunomodulatory mediator release during aging
    Authors: P Schädel, A Czapka, N Gebert, ID Jacobsen, A Ori, O Werz
    Aging Cell, 2023-04-26;0(0):e13856.  2023-04-26
  21. NOX4 as a critical effector mediating neuroinflammatory cytokines, myeloperoxidase and osteopontin, specifically in astrocytes in the hippocampus in Parkinson's disease
    Authors: N Boonpraman, S Yoon, CY Kim, JS Moon, SS Yi
    Redox Biology, 2023-04-10;62(0):102698.  2023-04-10
  22. Mesenchymal Stem Cells from COPD Patients Are Capable of Restoring Elastase-Induced Emphysema in a Murine Experimental Model
    Authors: C Río, AK Jahn, A Martin-Med, AM Calvo Bota, MT De Francis, PJ Pont Anton, O Gigirey Ca, ÁF Carvajal, C Villena Po, C Gómez Bell, A Iglesias, J Calvo Beni, A Gayà Puig, LA Ortiz, E Sala-Llinà
    International Journal of Molecular Sciences, 2023-03-18;24(6):.  2023-03-18
  23. Mice with endothelial cell-selective adhesion molecule deficiency develop coronary microvascular rarefaction and left ventricle diastolic dysfunction
    Authors: V Buncha, KA Fopiano, L Lang, C Williams, A Horuzsko, JA Filosa, G Kapuku, Z Bagi
    Physiological Reports, 2023-03-01;11(6):e15643.  2023-03-01
  24. Spectrin-Based Regulation of Cardiac Fibroblast Cell-Cell Communication
    Authors: DM Nassal, R Shaheen, NJ Patel, J Yu, N Leahy, D Bibidakis, NL Parinandi, TJ Hund
    Cells, 2023-02-26;12(5):.  2023-02-26
  25. Paracrine Signals in Calcified Conditioned Media Elicited Differential Responses in Primary Aortic Vascular Smooth Muscle Cells and in Adventitial Fibroblasts
    Authors: AM Kennon, JA Stewart
    International Journal of Molecular Sciences, 2023-02-10;24(4):.  2023-02-10
  26. The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIkappaBs) in Chronic Post-Ischemia Pain (CPIP) Models
    Authors: JS Chae, H Park, SH Ahn, EC Han, Y Lee, YJ Kim, EJ Ahn, HW Oh, HJ Lee, C Choi, YH Choi, WJ Kim
    Pharmaceutics, 2023-02-07;15(2):.  2023-02-07
  27. Chemotherapy-induced tumor immunogenicity is mediated in part by megakaryocyte-erythroid progenitors
    Authors: A Vorontsova, TJ Cooper, J Haj-Shomal, M Benguigui, S Levin, B Manobla, R Menachem, M Timaner, Z Raviv, Y Shaked
    Oncogene, 2023-01-16;0(0):.  2023-01-16
  28. Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration
    Authors: V Moiseeva, A Cisneros, V Sica, O Deryagin, Y Lai, S Jung, E Andrés, J An, J Segalés, L Ortet, V Lukesova, G Volpe, A Benguria, A Dopazo, S Aznar-Beni, Y Urano, A Del Sol, MA Esteban, Y Ohkawa, AL Serrano, E Perdiguero, P Muñoz-Cáno
    Nature, 2023;613(7942):169-178.  2023
  29. Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration
    Authors: V Moiseeva, A Cisneros, V Sica, O Deryagin, Y Lai, S Jung, E Andrés, J An, J Segalés, L Ortet, V Lukesova, G Volpe, A Benguria, A Dopazo, S Aznar-Beni, Y Urano, A Del Sol, MA Esteban, Y Ohkawa, AL Serrano, E Perdiguero, P Muñoz-Cáno
    Nature, 2022-12-21;613(7942):169-178.  2022-12-21
  30. Cannabidiol and Beta-Caryophyllene in Combination: A Therapeutic Functional Interaction
    Authors: H Blanton, L Yin, J Duong, K Benamar
    International Journal of Molecular Sciences, 2022-12-07;23(24):.  2022-12-07
  31. RNA binding protein HuD mediates the crosstalk between beta cells and islet endothelial cells by the regulation of Endostatin and Serpin E1 expression
    Authors: M Jung, S Ryu, C Kim, S Cha, H Kang, E Ji, Y Hong, Y Lee, S Han, SM Jeong, W Kim, EK Lee
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  32. MYC promotes immune-suppression in triple-negative breast cancer via inhibition of interferon signaling
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    Nat Commun, 2019-12-20;10(1):5816.  2019-12-20
  109. Depletion of Bone Marrow-Derived Fibrocytes Attenuates TAA-Induced Liver Fibrosis in Mice
    Authors: F Hempel, M Roderfeld, R Savai, A Sydykov, K Irungbam, R Schermuly, R Voswinckel, K Köhler, Y Churin, L Kiss, J Bier, J Pons-Kühne, E Roeb
    Cells, 2019-10-07;8(10):.  2019-10-07
  110. Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung
    Authors: J Schyns, Q Bai, C Ruscitti, C Radermecke, S De Scheppe, S Chakarov, F Farnir, D Pirottin, F Ginhoux, G Boeckxstae, F Bureau, T Marichal
    Nat Commun, 2019-09-03;10(1):3964.  2019-09-03
  111. Acidity promotes tumour progression by altering macrophage phenotype in prostate cancer
    Authors: A El-Kenawi, C Gatenbee, M Robertson-, R Bravo, J Dhillon, Y Balaguruna, A Berglund, N Visvakarma, A Ibrahim-Ha, J Choi, K Luddy, R Gatenby, S Pilon-Thom, A Anderson, B Ruffell, R Gillies
    Br. J. Cancer, 2019-08-16;121(7):556-566.  2019-08-16
  112. Tumour-Derived Laminin alpha5 (LAMA5) Promotes Colorectal Liver Metastasis Growth, Branching Angiogenesis and Notch Pathway Inhibition
    Authors: A Gordon-Wee, SY Lim, A Yuzhalin, S Lucotti, JAF Vermeer, K Jones, J Chen, RJ Muschel
    Cancers (Basel), 2019-05-06;11(5):.  2019-05-06
  113. Platelet GPIb? is a mediator and potential interventional target for NASH and subsequent liver cancer
    Authors: M Malehmir, D Pfister, S Gallage, M Szydlowska, D Inverso, E Kotsiliti, V Leone, M Peiseler, BGJ Surewaard, D Rath, A Ali, MJ Wolf, H Drescher, ME Healy, D Dauch, D Kroy, O Krenkel, M Kohlhepp, T Engleitner, A Olkus, T Sijmonsma, J Volz, C Deppermann, D Stegner, P Helbling, C Nombela-Ar, A Rafiei, M Hinterleit, M Rall, F Baku, O Borst, CL Wilson, J Leslie, T O'Connor, CJ Weston, DH Adams, L Sheriff, A Teijeiro, M Prinz, R Bogeska, N Anstee, MN Bongers, M Notohamipr, T Geisler, DJ Withers, J Ware, DA Mann, HG Augustin, A Vegiopoulo, MD Milsom, AJ Rose, PF Lalor, JM Llovet, R Pinyol, F Tacke, R Rad, M Matter, N Djouder, P Kubes, PA Knolle, K Unger, L Zender, B Nieswandt, M Gawaz, A Weber, M Heikenwald
    Nat. Med., 2019-04-01;25(4):641-655.  2019-04-01
  114. Liver-derived fibroblast growth factor 21 mediates effects of glucagon-like peptide-1 in attenuating hepatic glucose output
    Authors: J Liu, K Yang, J Yang, W Xiao, Y Le, F Yu, L Gu, S Lang, Q Tian, T Jin, R Wei, T Hong
    EBioMedicine, 2019-03-01;0(0):.  2019-03-01
  115. Prostate cancer promotes a vicious cycle of bone metastasis progression through inducing osteocytes to secrete GDF15 that stimulates prostate cancer growth and invasion
    Authors: W Wang, X Yang, J Dai, Y Lu, J Zhang, ET Keller
    Oncogene, 2019-02-12;0(0):.  2019-02-12
  116. Matrix bound nanovesicle-associated IL-33 activates a pro-remodeling macrophage phenotype via a non-canonical, ST2-independent pathway
    Authors: GS Hussey, JL Dziki, YC Lee, JG Bartolacci, M Behun, HR Turnquist, SF Badylak
    J Immunol Regen Med, 2019-02-01;3(0):26-35.  2019-02-01
  117. T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells
    Authors: HR Kim, Y Mun, KS Lee, YJ Park, JS Park, JH Park, BN Jeon, CH Kim, Y Jun, YM Hyun, M Kim, SM Lee, CS Park, SH Im, CD Jun
    Nat Commun, 2018-09-07;9(1):3630.  2018-09-07
  118. IL-23 secreted by myeloid cells drives castration-resistant prostate cancer
    Authors: A Calcinotto, C Spataro, E Zagato, D Di Mitri, V Gil, M Crespo, G De Bernard, M Losa, M Mirenda, E Pasquini, A Rinaldi, S Sumanasuri, MB Lambros, A Neeb, R Lucianò, CA Bravi, D Nava-Rodri, D Dolling, T Prayer-Gal, A Ferreira, A Briganti, A Esposito, S Barry, W Yuan, A Sharp, J de Bono, A Alimonti
    Nature, 2018-06-27;0(0):.  2018-06-27
  119. Comparison of the immune response during acute and chronic Staphylococcus aureus infection
    Authors: RA Brady, CP Mocca, RD Plaut, K Takeda, DL Burns
    PLoS ONE, 2018-03-29;13(3):e0195342.  2018-03-29
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    Authors: T Szatmári, E Persa, E Kis, A Benedek, R Hargitai, G Sáfrány, K Lumniczky
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FAQs

  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

  2. Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

    • We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

View all Proteome Profiler Antibody Array FAQs

Reviews for Proteome Profiler Mouse XL Cytokine Array

Average Rating: 4.9 (Based on 35 Reviews)

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Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 09/17/2024
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 05/23/2024

mouse liver lysates

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Mary-Kate Hayward on 04/05/2024

Performed on mammary tumor lysates.

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 06/29/2023

I bought two boxes, and they're both great

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 06/21/2023

easy to use kit

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Khuynh Nguyen on 05/06/2023

This product works very well

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Felipe Rodrigues on 10/16/2022

Conditioned medium of macrophages (36h) isolated from metastatic lungs.

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 08/28/2022

This kit is really great!

Proteome Profiler Mouse XL Cytokine Array
By Steven Nottley on 08/07/2022

Secretome of 4T1 murine breast cancer cells.

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Felipe Rodrigues on 08/07/2022

Cytokine array of the secretome of MLE12 cells.

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Dong Hu on 07/11/2022
Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 12/02/2021
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 10/21/2021
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 09/16/2021
Proteome Profiler Mouse XL Cytokine Array
By Soheil Saeedi on 08/10/2021

We used the array kit for profiling inflammatory cytokines in plasma samples from mice. I trust that it works very well.

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 03/14/2021
Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 09/17/2020

Excellent antibody array to screen a multitude of cytokines, chemokines, and growth factors in one small strip of membrane. Readout is not a fluorescence so you don't need a special fluorescence reader. If you are doing Western blotting, you can do this easily.

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 02/12/2020

Mouse breast tumor tissues

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 01/25/2020
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 01/06/2020

Excellent array!!

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 06/06/2019
Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 03/13/2019
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 12/05/2018
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 11/11/2018

Excellent specificity and detection for large number of cytokines. Works perfectly with little optimization.

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Monica Perez on 11/05/2018

Pancreas lysate. 750ug protein/ membrane

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 07/18/2018
Proteome Profiler Mouse XL Cytokine Array
By Leslie Priddy on 04/01/2018
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 03/22/2018
Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 12/07/2017

200 uL pooled serum from wild-type C57Bl/6 mice.
5 min exposure time

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Jakub Zmajkovic on 08/17/2017
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 07/17/2017
Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 05/23/2017
Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By James Beckett on 11/28/2016

150 uL of mouse serum

Proteome Profiler Mouse XL Cytokine Array ARY028
Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 09/30/2016

This is a great kit. It provides excellent reliability and a large number of cytokines, chemokines to screening in a single assay. I strongly recommend it.

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 08/26/2016

The sample was cell culture supernatant collected from MEF.

Proteome Profiler Mouse XL Cytokine Array ARY028